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Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research

Garschagen, Laura S. and Mancinelli, Rocco L. and Moeller, Ralf (2019) Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research. Astrobiology, 19 (10), pp. 1211-1220. Mary Ann Liebert Inc.. doi: 10.1089/ast.2018.2010. ISSN 1531-1074.

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2018.2010

Abstract

Microbial contamination of human-tended spacecraft is unavoidable, making the study of microbial growth under space conditions essential for the preservation of astronauts’ health and equipment integrity. Previous studies suggested that spaceflight conditions, such as microgravity, cause a range of physiological microbial alterations including increased growth yields and decreased antibiotic susceptibility. Because of its fast generation time, Vibrio natriegens could be used as a model organism for a variety of studies where generation time is a critical factor. In this study, V. natriegens was used as a tool to study growth characteristics by determining the viable cell number and antibiotic susceptibility under simulated microgravity using a 2-D clinostat (60 rpm) to establish a test system that resolves changes in microbial growth on a solid surface (agar) under microgravity. The data show that V. natriegens biomass increases significantly after 24 h at 37C under simulated microgravity. The final cell population after cultivation under simulated microgravity was 60-fold greater than when cultivated under normal terrestrial gravity (1 x g). No change in susceptibility to the antibiotic rifampicin after cultivation under simulated microgravity or normal gravity was detected. These data show that V. natriegens is a new and innovative model organism for microbial microgravity research.

Item URL in elib:https://elib.dlr.de/129123/
Document Type:Article
Title:Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Garschagen, Laura S.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany and University of Bonn, Institute for Microbiology and Biotechnology (IfMB), Bonn, Germany.UNSPECIFIED
Mancinelli, Rocco L.NASA Ames Research Center, Bay Area Environmental Research Institute, Moffett Field, California, USA.UNSPECIFIED
Moeller, RalfRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Ralf.Moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:5 September 2019
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1089/ast.2018.2010
Page Range:pp. 1211-1220
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Vibrio natriegens, Simulated microgravity, Clinostat, Growth, Antibiotic susceptibility
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:20 Sep 2019 11:34
Last Modified:08 Oct 2019 09:05

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