Garschagen, Laura S. und Mancinelli, Rocco L. und Moeller, Ralf (2019) Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research. Astrobiology, 19 (10), Seiten 1211-1220. Mary Ann Liebert Inc.. doi: 10.1089/ast.2018.2010. ISSN 1531-1074.
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Offizielle URL: http://dx.doi.org/10.1089/ast.2018.2010
Kurzfassung
Microbial contamination of human-tended spacecraft is unavoidable, making the study of microbial growth under space conditions essential for the preservation of astronauts’ health and equipment integrity. Previous studies suggested that spaceflight conditions, such as microgravity, cause a range of physiological microbial alterations including increased growth yields and decreased antibiotic susceptibility. Because of its fast generation time, Vibrio natriegens could be used as a model organism for a variety of studies where generation time is a critical factor. In this study, V. natriegens was used as a tool to study growth characteristics by determining the viable cell number and antibiotic susceptibility under simulated microgravity using a 2-D clinostat (60 rpm) to establish a test system that resolves changes in microbial growth on a solid surface (agar) under microgravity. The data show that V. natriegens biomass increases significantly after 24 h at 37C under simulated microgravity. The final cell population after cultivation under simulated microgravity was 60-fold greater than when cultivated under normal terrestrial gravity (1 x g). No change in susceptibility to the antibiotic rifampicin after cultivation under simulated microgravity or normal gravity was detected. These data show that V. natriegens is a new and innovative model organism for microbial microgravity research.
elib-URL des Eintrags: | https://elib.dlr.de/129123/ | ||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||
Titel: | Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research | ||||||||||||||||
Autoren: |
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Datum: | 5 September 2019 | ||||||||||||||||
Erschienen in: | Astrobiology | ||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||
Open Access: | Nein | ||||||||||||||||
Gold Open Access: | Nein | ||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||
Band: | 19 | ||||||||||||||||
DOI: | 10.1089/ast.2018.2010 | ||||||||||||||||
Seitenbereich: | Seiten 1211-1220 | ||||||||||||||||
Verlag: | Mary Ann Liebert Inc. | ||||||||||||||||
ISSN: | 1531-1074 | ||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||
Stichwörter: | Vibrio natriegens, Simulated microgravity, Clinostat, Growth, Antibiotic susceptibility | ||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Vorhaben Strahlenbiologie (alt) | ||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie | ||||||||||||||||
Hinterlegt von: | Kopp, Kerstin | ||||||||||||||||
Hinterlegt am: | 20 Sep 2019 11:34 | ||||||||||||||||
Letzte Änderung: | 31 Okt 2023 13:56 |
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