elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Geomicrobiology of a seawater-influenced active sulfuric acid cave

D’Angeli, Ilenia M. and Ghezzi, Daniele and Leuko, Stefan and Firrincieli, Andrea and Parise, Mario and Fiorucci, Adriano and Vigna, Bartolomeo and Addesso, Rosangela and Baldantoni, Daniela and Carbone, Cristina and Miller, Ana Zelia and Jurado, Valme and Saiz-Jimenez, Cesareo and De Waele, Jo and Cappelletti, Martina (2019) Geomicrobiology of a seawater-influenced active sulfuric acid cave. PLoS One, 14 (8), e0220706. Public Library of Science (PLoS). DOI: 10.1371/journal.pone.0220706 ISSN 1932-6203

[img] PDF - Published version
5MB

Official URL: http://dx.doi.org/10.1371/journal.pone.0220706

Abstract

Fetida Cave is an active sulfuric acid cave influenced by seawater, showing abundant microbial communities that organize themselves under three main different morphologies: water filaments, vermiculations and moonmilk deposits. These biofilms/deposits have different cave distribution, pH, macro- and microelement and mineralogical composition, carbon and nitrogen content. In particular, water filaments and vermiculations had circumneutral and slightly acidic pH, respectively, both had abundant organic carbon and high microbial diversity. They were rich in macro- and microelements, deriving from mineral dissolution, and, in the case of water filaments, from seawater composition. Vermiculations had different color, partly associated with their mineralogy, and unusual minerals probably due to trapping capacities. Moonmilk was composed of gypsum, poor in organic matter, had an extremely low pH (0–1) and low microbial diversity. Based on 16S rRNA gene analysis, the microbial composition of the biofilms/deposits included autotrophic taxa associated with sulfur and nitrogen cycles and biomineralization processes. In particular, water filaments communities were characterized by bacterial taxa involved in sulfur oxidation and reduction in aquatic, aphotic, microaerophilic/anoxic environments (Campylobacterales, Thiotrichales, Arenicellales, Desulfobacterales, Desulforomonadales) and in chemolithotrophy in marine habitats (Oceanospirillales, Chromatiales). Their biodiversity was linked to the morphology of the water filaments and their collection site. Microbial communities within vermiculations were partly related to their color and showed high abundance of unclassified Betaproteobacteria and sulfur-oxidizing Hydrogenophilales (including Sulfuriferula), and Acidiferrobacterales (including Sulfurifustis), sulfur-reducing Desulfurellales, and ammonia-oxidizing Planctomycetes and Nitrospirae. The microbial community associated with gypsum moonmilk showed the strong dominance (>60%) of the archaeal genus Thermoplasma and lower abundance of chemolithotrophic Acidithiobacillus, metal-oxidizing Metallibacterium, Sulfobacillus, and Acidibacillus. This study describes the geomicrobiology of water filaments, vermiculations and gypsum moonmilk from Fetida Cave, providing insights into the microbial taxa that characterize each morphology and contribute to biogeochemical cycles and speleogenesis of this peculiar seawater-influenced sulfuric acid cave.

Item URL in elib:https://elib.dlr.de/129037/
Document Type:Article
Title:Geomicrobiology of a seawater-influenced active sulfuric acid cave
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
D’Angeli, Ilenia M.Department of Biological, Geological and Environmental Sciences, University of Bologna, Bologna, Italy,UNSPECIFIED
Ghezzi, DanieleDepartment of Pharmacy and Biotechnology, University of Bologna, Bologna, ItalyUNSPECIFIED
Leuko, StefanRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.https://orcid.org/0000-0002-0423-0896
Firrincieli, AndreaSchool of Environmental and Forest Science, University of Washington, Seattle, WA, United States of AmericaUNSPECIFIED
Parise, MarioDepartment of Geological and Environmental Sciences, University of Bari “Aldo Moro”, Bari, ItalyUNSPECIFIED
Fiorucci, AdrianoDepartment of Environment, Land and Infrastructure Engineering, Polytechnic University of Turin, Torino, ItalyUNSPECIFIED
Vigna, BartolomeoDepartment of Environment, Land and Infrastructure Engineering, Polytechnic University of Turin, Torino, ItalyUNSPECIFIED
Addesso, RosangelaDepartment of Chemistry and Biology “Adolfo Zambelli”, University of Salerno, Fisciano (SA), ItalyUNSPECIFIED
Baldantoni, DanielaDepartment of Chemistry and Biology “Adolfo Zambelli”, University of Salerno, Fisciano (SA), ItalyUNSPECIFIED
Carbone, CristinaDISTAV, Department of Geological, Environmental and Biological Sciences, University of Genoa, Genoa, ItalyUNSPECIFIED
Miller, Ana ZeliaHERCULES Laboratory, University of Évora, Èvora, PortugalUNSPECIFIED
Jurado, ValmeInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Sevilla, SpainUNSPECIFIED
Saiz-Jimenez, CesareoInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Sevilla, SpainUNSPECIFIED
De Waele, JoDepartment of Biological, Geological and Environmental Sciences, University of Bologna, Bologna, Italy,UNSPECIFIED
Cappelletti, MartinaDepartment of Pharmacy and Biotechnology, University of Bologna, Bologna, ItalyUNSPECIFIED
Date:8 August 2019
Journal or Publication Title:PLoS One
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:14
DOI :10.1371/journal.pone.0220706
Page Range:e0220706
Editors:
EditorsEmail
Senko, John M.The University of Akron, UNITED STATES
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Status:Published
Keywords:Geomicrobiology, cave, microbial community,
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:20 Sep 2019 11:34
Last Modified:20 Sep 2019 11:34

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.