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Parabolic Flight-Induced Acute Hypergravity and Microgravity Modulate the Beating Contractile Rate of Human Cardiomyocytes

Acharya, Aviseka and Brungs, Sonja and Lichterfeld, Yannick and Hescheler, Jürgen and Hemmersbach, Ruth and Boeuf, Helene and Sachinidis, Apapios (2019) Parabolic Flight-Induced Acute Hypergravity and Microgravity Modulate the Beating Contractile Rate of Human Cardiomyocytes. Cells, 8 (352), pp. 1-11. Multidisciplinary Digital Publishing Institute (MDPI). DOI: 10.3390/cells8040352 ISSN 2073-4409

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Official URL: https://doi.org/10.3390/cells8040352

Abstract

Functional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects of altered gravity on hCMs, we exposed them to acute hypergravity and microgravity phases in the presence and absence of the beta-adrenoceptor isoprenalin (ISO), L-type Ca2-plus channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, and monitored their beating rate (BR). These logistically demanding experiments were executed during the 66th Parabolic Flight Campaign of the European Space Agency. The hCM cultures were exposed to 31 alternating hypergravity, microgravity, and hypergravity phases, each lasting 20 to 22 s. During the parabolic flight experiment, BR and cell viability were monitored using the xCELLigence real-time cell analyzer Cardio Instrument (trademark). Corresponding experiments were performed on the ground (1 g), using an identical set-up. Our results showed that BR continuously increased during the parabolic flight, reaching a 40% maximal increase after 15 parabolas, compared with the pre-parabolic (1 g) phase. However, in the presence of the LTCC blocker nifedipine, no change in BR was observed, even after 31 parabolas. We surmise that the parabola-mediated increase in BR was induced by the LTCC blocker. Moreover, the increase in BR induced by ISO and Bay-K8644 during the pre-parabola phase was further elevated by 20% after 25 parabolas. This additional effect reflects the positive impact of the parabolas in the absence of both agonists. Our study suggests that acute alterations of gravity significantly increase the BR of hCMs via the LTCC.

Item URL in elib:https://elib.dlr.de/128557/
Document Type:Article
Title:Parabolic Flight-Induced Acute Hypergravity and Microgravity Modulate the Beating Contractile Rate of Human Cardiomyocytes
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Acharya, AvisekaUniversity of Cologne, Institute of Neurophysiology and center for molecular medicine cologne (cmmc), Cologne, Germanyhttps://orcid.org/0000-0003-4110-4968
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920
Hescheler, Jürgenuniversity of cologne, institute of neurophysiology and center for molecular medicine cologne (cmmc), cologne, germanyhttps://orcid.org/0000-0001-7686-6562
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715
Boeuf, HeleneINSERM (French National Institute of Health and Medical Research), Université de Bordeaux, Francehttps://orcid.org/0000-0002-3006-8773
Sachinidis, ApapiosUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, Germanyhttps://orcid.org/0000-0003-3054-225X
Date:14 April 2019
Journal or Publication Title:Cells
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:No
In ISI Web of Science:Yes
Volume:8
DOI :10.3390/cells8040352
Page Range:pp. 1-11
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2073-4409
Status:Published
Keywords:Microgravity, hypergravity, human induced pluripotent stem cells, cardiomyocytes, adrenoceptor agonist, Isoprenaline, L-type Ca2plus channels, Bay-K8644
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:14 Aug 2019 10:00
Last Modified:14 Dec 2019 04:25

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