Acharya, Aviseka und Brungs, Sonja und Lichterfeld, Yannick und Hescheler, Jürgen und Hemmersbach, Ruth und Boeuf, Helene und Sachinidis, Apapios (2019) Parabolic Flight-Induced Acute Hypergravity and Microgravity Modulate the Beating Contractile Rate of Human Cardiomyocytes. Cells, 8 (4), Seite 352. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/cells8040352. ISSN 2073-4409.
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Offizielle URL: https://doi.org/10.3390/cells8040352
Kurzfassung
Functional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects of altered gravity on hCMs, we exposed them to acute hypergravity and microgravity phases in the presence and absence of the beta-adrenoceptor isoprenalin (ISO), L-type Ca2-plus channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, and monitored their beating rate (BR). These logistically demanding experiments were executed during the 66th Parabolic Flight Campaign of the European Space Agency. The hCM cultures were exposed to 31 alternating hypergravity, microgravity, and hypergravity phases, each lasting 20 to 22 s. During the parabolic flight experiment, BR and cell viability were monitored using the xCELLigence real-time cell analyzer Cardio Instrument (trademark). Corresponding experiments were performed on the ground (1 g), using an identical set-up. Our results showed that BR continuously increased during the parabolic flight, reaching a 40% maximal increase after 15 parabolas, compared with the pre-parabolic (1 g) phase. However, in the presence of the LTCC blocker nifedipine, no change in BR was observed, even after 31 parabolas. We surmise that the parabola-mediated increase in BR was induced by the LTCC blocker. Moreover, the increase in BR induced by ISO and Bay-K8644 during the pre-parabola phase was further elevated by 20% after 25 parabolas. This additional effect reflects the positive impact of the parabolas in the absence of both agonists. Our study suggests that acute alterations of gravity significantly increase the BR of hCMs via the LTCC.
elib-URL des Eintrags: | https://elib.dlr.de/128557/ | ||||||||||||||||||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||||||||||||||
Titel: | Parabolic Flight-Induced Acute Hypergravity and Microgravity Modulate the Beating Contractile Rate of Human Cardiomyocytes | ||||||||||||||||||||||||||||||||
Autoren: |
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Datum: | 14 April 2019 | ||||||||||||||||||||||||||||||||
Erschienen in: | Cells | ||||||||||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||||||||||
Open Access: | Ja | ||||||||||||||||||||||||||||||||
Gold Open Access: | Ja | ||||||||||||||||||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||||||||||||||||||
Band: | 8 | ||||||||||||||||||||||||||||||||
DOI: | 10.3390/cells8040352 | ||||||||||||||||||||||||||||||||
Seitenbereich: | Seite 352 | ||||||||||||||||||||||||||||||||
Verlag: | Multidisciplinary Digital Publishing Institute (MDPI) | ||||||||||||||||||||||||||||||||
ISSN: | 2073-4409 | ||||||||||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||||||||||
Stichwörter: | Microgravity, hypergravity, human induced pluripotent stem cells, cardiomyocytes, adrenoceptor agonist, Isoprenaline, L-type Ca2plus channels, Bay-K8644 | ||||||||||||||||||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (alt), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt) | ||||||||||||||||||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie | ||||||||||||||||||||||||||||||||
Hinterlegt von: | Duwe, Helmut | ||||||||||||||||||||||||||||||||
Hinterlegt am: | 14 Aug 2019 10:00 | ||||||||||||||||||||||||||||||||
Letzte Änderung: | 31 Okt 2020 04:35 |
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