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Entwicklung eines halbautomatischen Programms zur bildanalytischen Auswertung der Morphologie von Neuronen mittels Sholl-Analyse

Bareth, Marlo (2019) Entwicklung eines halbautomatischen Programms zur bildanalytischen Auswertung der Morphologie von Neuronen mittels Sholl-Analyse. Bachelor's, University of Applied Sciences, Hochschule Emden-Leer.

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Abstract

This thesis deals with the development of a program for the semi-automatic analysis of (fluorescence) microscopic images. Sholl analysis is used to investigate the morphological complexity of neurons that are altered by neurotoxic influences such as certain agents or radiation. The latter is of importance in the risk assessment of interplanetary travel of humans. The developed application has a complete graphical user interface and the possibility to document the analysed images with data from the experiments. For image processing, the OpenCV libraries were used. The images are first segmented. The origins of the outgrowing neurites can be determined by a developed method. Originating from these somatic starting points they are traced with a ChainCodebased algorithm. The implemented Sholl analysis uses mathematical formulas to determine the number of intersections between neurites and a circle whose centre lies in the soma of the cell. The radii and step sizes required for this can be given in pixels or µm. To simplify the conversion, CZI files can be loaded from which the corresponding metadata can be imported. Each intermediate step can be saved as an image and the analysis results are exported as a CSV file.

Item URL in elib:https://elib.dlr.de/128237/
Document Type:Thesis (Bachelor's)
Title:Entwicklung eines halbautomatischen Programms zur bildanalytischen Auswertung der Morphologie von Neuronen mittels Sholl-Analyse
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Bareth, MarloRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Date:May 2019
Refereed publication:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Number of Pages:74
Status:Published
Keywords:Sholl-Analyse, Neuronen, Morphologie
Institution:University of Applied Sciences, Hochschule Emden-Leer
Department:Fachbereich Technik, Abteilung naturwissenschaftliche Technik
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:03 Jul 2019 13:02
Last Modified:03 Jul 2019 13:02

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