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Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

Coussot, Gaëlle and Le Postollec, Aurélie and Incerti, Sébastien and Baqué, Mickaël and Faye, Clément and Vandenabeele-Trambouze, Odile and Cottin, Hervé and Ravelet, Corinne and Peyrin, Eric and Fiore, Emmanuelle and Vigier, Flavie and Caron, Jérôme and Chaput, Didier and Przybyla, Bartos and Berger, Thomas and Dobrijevic, Michel (2019) Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station. Astrobiology, 19 (8), pp. 1063-1074. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2018.1896 ISSN 1531-1074

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Official URL: http://dx.doi.org/10.1089/ast.2018.1896

Abstract

Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several groundbased facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSER2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during shortterm exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21°C to +58°C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSER2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.

Item URL in elib:https://elib.dlr.de/128233/
Document Type:Article
Title:Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Coussot, GaëlleInstitut des Biomolécules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, Montpellier, France.UNSPECIFIED
Le Postollec, AurélieLaboratoire d’Astrophysique de Bordeaux (LAB), Université de Bordeaux, CNRS, B18N, Pessac, France.UNSPECIFIED
Incerti, SébastienCentre d’Etudes Nucléaires de Bordeaux Gradignan (CENBG), UMR 5797, Université de Bordeaux, Gradignan, France.UNSPECIFIED
Baqué, MickaëlGerman Aerospace Center (DLR), Institute of Planetary Research, Management and Infrastructure, Research Group Astrobiological Laboratories, Berlin, Germany.https://orcid.org/0000-0002-6696-6030
Faye, ClémentCap Alpha, Colcom, Clapiers, FranceUNSPECIFIED
Vandenabeele-Trambouze, OdileIUEM—UMR 6197, Laboratoire de Microbiologie des Environnements Extreˆmes (LMEE), Université de Bretagne Occidentale (UBO), Plouzané, France.UNSPECIFIED
Cottin, HervéLaboratoire Interuniversitaire des Syste`mes Atmosphe´riques (LISA), UMR 7583, Université Paris Est Créteil et Université Paris Diderot, Institut Pierre Simon Laplace, Créteil, France.UNSPECIFIED
Ravelet, CorinneDépartement de Pharmacochimie Mole´culaire, UMR 5063, Université Grenoble Alpes, CNRS, St. Martin d’Héres, France.UNSPECIFIED
Peyrin, EricDépartement de Pharmacochimie Mole´culaire, UMR 5063, Université Grenoble Alpes, CNRS, St. Martin d’Héres, France.UNSPECIFIED
Fiore, EmmanuelleDépartement de Pharmacochimie Mole´culaire, UMR 5063, Université Grenoble Alpes, CNRS, St. Martin d’Héres, France.UNSPECIFIED
Vigier, Flavie*This coauthor is now working for private companies.UNSPECIFIED
Caron, JérômeDépartement de Radiothérapie, Institut Bergonié, Comprehensive Cancer Center, Bordeaux, France.UNSPECIFIED
Chaput, DidierDCT/ME/EM, Centre National d’Etudes Spatiales, Toulouse, France.UNSPECIFIED
Przybyla, BartosRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyUNSPECIFIED
Berger, ThomasRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Thomas.Berger (at) dlr.dehttps://orcid.org/0000-0003-3319-5740
Dobrijevic, MichelLaboratoire d’Astrophysique de Bordeaux (LAB), Universite´ de Bordeaux, CNRS, B18N, Pessac, France.UNSPECIFIED
Date:28 February 2019
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1089/ast.2018.1896
Page Range:pp. 1063-1074
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Astrobiology, Cosmic rays, Biochip, Aptamers
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie, R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Planetary Research > Leitungsbereich PF
Deposited By: Kopp, Kerstin
Deposited On:03 Jul 2019 13:01
Last Modified:16 Sep 2019 14:27

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