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Photochemistry on the Space Station—Antibody Resistance to Space Conditions after Exposure outside the International Space Station

Coussot, Gaëlle and Le Postollec, Aurélie and Faye, Clément and Baqué, Mickaël and Vandenabeele-Trambouze, Odile and Incerti, Sébastien and Vigier, Flavie and Chaput, Didier and Cottin, Hervé and Przybyla, Bartos and Berger, Thomas and Dobrijevic, Michel (2019) Photochemistry on the Space Station—Antibody Resistance to Space Conditions after Exposure outside the International Space Station. Astrobiology, 19 (8), pp. 1053-1062. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2018.1907 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2018.1907

Abstract

Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy) corresponded to the absorbed dose expected during a mission to Mars. Moreover, samples faced, at the same time as irradiation, thermal cycles, launch constraints, and long-term storage. A model biochip was used in this study with antibodies in freeze-dried form and under two formats: free or covalently grafted to a solid surface. We found that antibody-binding performances were not significantly affected by cosmic radiation, and more than 40% of the exposed antibody, independent of its format, was still functional during all this experiment. We conclude that antibody-based instruments are well suited for in situ analysis on planetary bodies.

Item URL in elib:https://elib.dlr.de/128232/
Document Type:Article
Title:Photochemistry on the Space Station—Antibody Resistance to Space Conditions after Exposure outside the International Space Station
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Coussot, GaëlleInstitut des Biomolécules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, Montpellier, FranceUNSPECIFIED
Le Postollec, AurélieLaboratoire d’Astrophysique de Bordeaux (LAB), Université de Bordeaux, CNRS, Pessac, France.UNSPECIFIED
Faye, ClémentColcom, Cap Alpha, Clapiers, France.UNSPECIFIED
Baqué, MickaëlGerman Aerospace Center (DLR), Institute of Planetary Research, Management and Infrastructure, Research Group Astrobiological Laboratories, Berlin, Germany.https://orcid.org/0000-0002-6696-6030
Vandenabeele-Trambouze, OdileUniversité de Bretagne Occidentale (UBO), IUEM—UMR 6197, Laboratoire de Microbiologie des Environnements Extrémes (LMEE), Plouzané, France.UNSPECIFIED
Incerti, SébastienCentre d’Etudes Nucléaires de Bordeaux Gradignan (CENBG), UMR 5797, Université de Bordeaux, Gradignan, France.UNSPECIFIED
Vigier, Flavie*This coauthor is now working for private companies.UNSPECIFIED
Chaput, DidierCentre National d’Etudes Spatiales, DCT/ME/EM, Toulouse, France.UNSPECIFIED
Cottin, HervéLaboratoire Interuniversitaire des Systémes Atmosphériques (LISA), UMR 7583, Université Paris Est Créteil et Université Paris Diderot, Institut Pierre Simon Laplace, Créteil, France.UNSPECIFIED
Przybyla, BartosDiderot, Institut Pierre Simon Laplace, Créteil, France.UNSPECIFIED
Berger, ThomasRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Thomas.Berger (at) dlr.dehttps://orcid.org/0000-0003-3319-5740
Dobrijevic, MichelLaboratoire d’Astrophysique de Bordeaux (LAB), Université de Bordeaux, CNRS, Pessac, France.UNSPECIFIED
Date:28 February 2019
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1089/ast.2018.1907
Page Range:pp. 1053-1062
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Astrobiology, Cosmic rays, Biochip, Antibody, Planetary exploration
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems, R - Vorhaben Strahlenbiologie
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:03 Jul 2019 13:00
Last Modified:18 Sep 2019 12:27

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