Aggregated Cell Masses Provide Protection against Space Extremes and a Microhabitat for Hitchhiking Co-Inhabitants

Abstract

The European Space Agency’s EXPOSE facility, located on the outside of the International Space Station, was used to investigate the survival of cell aggregates of a cyanobacterium, Gloeocapsa sp., in space and simulated martian conditions for 531 days in low Earth orbit as part of the ‘‘Biofilm Organisms Surfing Space’’ (BOSS) experiments. Postflight analysis showed that the cell aggregates of the organism conferred protection against space conditions compared to planktonic cells. These cell aggregates, which consisted of groups of metabolically inactive cells that do not form structured layered biofilms, demonstrated that disordered ‘‘primitive’’ aggregates of sheathed cells can provide protection against environmental stress such as UV radiation. Furthermore, the experiment demonstrated that the cyanobacterial cell aggregates provided a microhabitat for a smaller bacterial co-cultured species that also survived in space. This observation shows that viable cells can ‘‘hitchhike’’ through space within the confines of larger protecting cells or cell aggregates, with implications for planetary protection, human health, and other space microbiology applications.