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Mitochondrial adaptations in elderly and young men skeletal muscle following two weeks of bed rest and rehabilitation

Buso, Alessia and Comelli, Marina and Picco, Raffaella and Isola, Miriam and Magnesa, Benedetta and Pisot, Rado and Rittweger, Jörn and Salvadego, Desy and Simunic, Boštjan and Grassi, Bruno and Mavelli, Irene (2019) Mitochondrial adaptations in elderly and young men skeletal muscle following two weeks of bed rest and rehabilitation. Frontiers in Physiology, 10 (474). Frontiers Media S.A. DOI: 10.3389/fphys.2019.00474 ISSN 1664-042X

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Abstract

The aim of the study was to evaluate the expression levels of proteins related to mitochondrial biogenesis regulation and bioenergetics in vastus lateralis muscle biopsies from 16 elderly and 7 young people subjected to 14 days of bed-rest, causing atrophy, and subsequent 14 days of exercise training. Based on quantitative immunoblot analyses, in both groups a reduction of two key regulators of mitochondrial biogenesis/remodeling and activity, namely PGC-1α and Sirt3, was revealed during bed-rest, with a subsequent up-regulation after rehabilitation, indicating an involvement of PGC-1α-Sirt3 axis in response to the treatments. A difference was observed comparing the young and elderly subjects as, for both proteins, the abundance in the elderly was more affected by immobility and less responsive to exercise. The expression levels of TOM20 and Citrate Synthase, assayed as markers of outer mitochondrial membrane and mitochondrial mass, showed a noticeable sensitivity in the elderly group, where they were affected by bed-rest and rehabilitation recalling the pattern of PGC-1α. TOM20 and CS remained unchanged in young subjects. Single OXPHOS complexes showed peculiar patterns, which were in some cases dissimilar from PGC-1α, and suggest different influences on protein biogenesis and degradation. Overall, exercise was capable to counteract the effect of immobility, when present, except for complex V, which was markedly downregulated by bed-rest, but remained unaffected after rehabilitation, maybe as result of greater extent of degradation processes over biogenesis. Phosphorylation extent of AMPK, and its upstream activator LKB1, did not change after bed-rest and rehabilitation in either young or elderly subjects, suggesting that the activation of energy-sensing LKB1-AMPK signaling pathway was “missed” due to its transient nature, or was not triggered under our conditions. Our study demonstrates that, as far as the expression of various proteins related to mitochondrial biogenesis/remodeling, adaptations to bed-rest and rehabilitation in the two populations were different. The impact of bed-rest was greater in the elderly subjects, where the pattern (decrease after bed rest and recovery following rehabilitation) was accompanied by changes of mitochondrial mass. Modifications of protein abundance were matched with data obtained from gene expression analyses of four public human datasets focusing on related genes.

Item URL in elib:https://elib.dlr.de/127435/
Document Type:Article
Title:Mitochondrial adaptations in elderly and young men skeletal muscle following two weeks of bed rest and rehabilitation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Buso, AlessiaDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Comelli, MarinaDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Picco, RaffaellaDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Isola, MiriamDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Magnesa, BenedettaDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Pisot, RadoUniversity of PrimorskaUNSPECIFIED
Rittweger, JörnJoern.Rittweger (at) dlr.dehttps://orcid.org/0000-0002-2223-8963
Salvadego, DesyDepartment of Medicine, University of Udine, Udine, ItalyUNSPECIFIED
Simunic, BoštjanUniversity of Primorska, SloveniaUNSPECIFIED
Grassi, BrunoUniversity of Udine, ItalyUNSPECIFIED
Mavelli, IreneUniversity of UdineUNSPECIFIED
Date:1 May 2019
Journal or Publication Title:Frontiers in Physiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI :10.3389/fphys.2019.00474
Publisher:Frontiers Media S.A
ISSN:1664-042X
Status:Published
Keywords:mitochondrial; biogenesis; skeletal muscle;
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Systemphysiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Becker, Christine
Deposited On:23 May 2019 12:09
Last Modified:23 May 2019 12:33

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