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Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions

Ott, Emanuel und Kawaguchi, Yuko und Özgen, Natalie und Yamagishi, Akihiko und Rabbow, Elke und Rettberg, Petra und Weckwerth, Wolfram und Milojevic, Tetyana (2019) Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions. Frontiers in Microbiology, 10, Seite 909. Frontiers Media S.A.. doi: 10.3389/fmicb.2019.00909. ISSN 1664-302X.

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Offizielle URL: http://dx.doi.org/10.3389/fmicb.2019.00909

Kurzfassung

The polyextremophile, gram-positive bacterium Deinococcus radiodurans can withstand harsh conditions of real and simulated outer space environment, e.g., UV and ionizing radiation. A long-term space exposure of D. radiodurans has been performed in Low Earth Orbit (LEO) in frames of the Tanpopo orbital mission aiming to investigate the possibility of interplanetary life transfer. Space vacuum (10⁻⁴–10⁻⁷ Pa) is a harmful factor, which induces dehydration and affects microbial integrity, severely damaging cellular components: lipids, carbohydrates, proteins, and nucleic acids. However, the molecular strategies by which microorganisms protect their integrity on molecular and cellular levels against vacuum damage are not yet understood. In a simulation experiment, we exposed dried D. radiodurans cells to vacuum (10⁻⁴–10⁻⁷ Pa), which resembles vacuum pressure present outside the International Space Station in LEO. After 90 days of high vacuum exposure, survival of D. radiodurans cells was 2.5-fold lower compared to control cells. To trigger molecular repair mechanisms, vacuum exposed cells of D. radiodurans were recovered in complex medium for 3 and 6 h. The combined approach of analyzing primary metabolites and proteins revealed important molecular activities during early recovery after vacuum exposure. In total, 1939 proteins covering 63% of D. radiodurans annotated protein sequences were detected. Proteases, tRNA ligases, reactive oxygen species (ROS) scavenging proteins, nucleic acid repair proteins, TCA cycle proteins, and S-layer proteins are highly abundant after vacuum exposure. The overall abundance of amino acids and TCA cycle intermediates is reduced during the recovery phase of D. radiodurans as they are needed as carbon source. Furthermore, vacuum exposure induces an upregulation of Type III histidine kinases, which trigger the expression of S-layer related proteins. Along with the highly abundant transcriptional regulator of FNR/CRP family, specific histidine kinases might be involved in the regulation of vacuum stress response. After repair processes are finished, D. radiodurans switches off the connected repair machinery and focuses on proliferation. Combined comparative analysis of alterations in the proteome and metabolome helps to identify molecular key players in the stress response of D. radiodurans, thus elucidating the mechanisms behind its extraordinary regenerative abilities and enabling this microorganism to withstand vacuum stress.

elib-URL des Eintrags:https://elib.dlr.de/127304/
Dokumentart:Zeitschriftenbeitrag
Titel:Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Ott, EmanuelDepartment of Biophysical Chemistry, University of Vienna, Vienna, AustriaNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Kawaguchi, YukoPlanetary Exploration Research Center (PERC), Chiba Institute of Technology (CIT), Chiba, JapanNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Özgen, NatalieDepartment of Biophysical Chemistry, University of Vienna, Vienna, AustriaNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Yamagishi, AkihikoDepartment of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta, Yokohama, JapanNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021NICHT SPEZIFIZIERT
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395NICHT SPEZIFIZIERT
Weckwerth, WolframDepartment of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria and Vienna Metabolomics Center (VIME), University of Vienna, Vienna, AustriaNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Milojevic, TetyanaDepartment of Biophysical Chemistry, University of Vienna, Vienna, Austria; tetyana.milojevic (at) univie.ac.atNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Datum:29 April 2019
Erschienen in:Frontiers in Microbiology
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:10
DOI:10.3389/fmicb.2019.00909
Seitenbereich:Seite 909
Verlag:Frontiers Media S.A.
ISSN:1664-302X
Status:veröffentlicht
Stichwörter:Deinococcus radiodurans, high vacuum exposure, dehydration, proteomics, metabolomics, molecular stress response
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:09 Mai 2019 13:13
Letzte Änderung:30 Sep 2020 16:10

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