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Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

Maleki, Hajar and Susan, Montes and Mohammad-Ali, Shahbazi and Hojjat Hosseini, Seyed and Reza Eskandari, Mohammad and Zaunschirm, Stefan and Verwanger, Thomas and Mathur, Sanjay and Milow, Barbara and Krammer, Barbara and Hüsing, Nicola (2019) Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration. ACS Applied Materials and Interfaces. American Chemical society (ACS). ISSN 1944-8244.

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Abstract

Due to the synergic feature of individual components in hybrid (nano)biomaterials, their application in regenerative medicine has drawn a significant attention. Aiming to address all the current challenges of aerogel as a potent scaffold in bone tissue engineering application, we adopted a novel synthesis approach to synergistically improve the pore size regime and mechanical strength in the aerogel. The 3D aerogel scaffold in this study has been synthesized through a versatile one-pot aqueous based sol-gel hybridization/assembly of organosilane tetraethylorthosilicate, TEOS) and silk fibroin (SF) biopolymer followed by unidirectional freeze casting the as-prepared hybrid gel and supercritical drying. The developed ultra-light silica-SF aerogel hybrids demonstrated a hierarchically organized porous structure with interesting honeycomb shaped micromorphology and microstructural alignment (anisotropy) in varied length scales. The average macropore size of hybrid aerogel lied in 0.5-18 micrometer and was systematically controlled with freezecasting conditions. Together with high porosity (91-94%), high Youngs modulus (~ 4-7 MPa, > 3 order of magnitude improvement compared to their pristine aerogel counterparts), and bone-type anisotropy in the mechanical compressive behaviour, the silica-SF hybrid aerogel of this study acted as a very competent scaffold for bone tissue formation. Namely, the results of in vitro assessments revealed that silica-SF aerogel is not only cytocompatible and non-hemolytic but also acted as an open porous microenvironment to trigger the osteoblast cells attachment, growth and proliferation on its surface within 14 days of incubation. Moreover, in order to support the in vitro results, in vivo bone formation within the aerogels implant in the bone defect site has been studied. The X-ray radiology and micro-CT analyses confirmed a significant new bone tissue density formed in the defect site within 25days of implantation. Also, in vivo toxicology studies showed a zero-toxic impact of aerogel implant on the blood biochemical and hematological parameters. Finally, the study clearly shows the potential of aerogel as a bioactive and osteoconductive open porous cellular matrix for successful osseointegration process.

Item URL in elib:https://elib.dlr.de/127256/
Document Type:Article
Title:Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Maleki, Hajarh.maleki (at) uni-koeln.deUNSPECIFIED
Susan, MontesDepartment of Chemistry and Physics of Materials, Paris-Lodron University of Salzburg, AustriaUNSPECIFIED
Mohammad-Ali, ShahbaziDepartment of Micro‐ and Nanotechnology, Technical University of Denmark, DK‐2800UNSPECIFIED
Hojjat Hosseini, SeyedDepartment of Pharmacology, School of Medicine, Zanjan University of MedicalUNSPECIFIED
Reza Eskandari, MohammadDepartment of Pharmacology and Toxicology, School of Pharmacy, Zanjan University ofUNSPECIFIED
Zaunschirm, StefanUniversity of Applied Sciences Upper Austria, Franz-Fritsch-Straße 11, 4600, Wels, AustriaUNSPECIFIED
Verwanger, ThomasDepartment of Biosciences, Paris-Lodron University Salzburg, Hellbrunnerstr. 34, 5020, Salzburg, AustriaUNSPECIFIED
Mathur, Sanjaysanjay.mathur (at) uni-koeln.deUNSPECIFIED
Milow, BarbaraBarbara.Milow (at) dlr.dehttps://orcid.org/0000-0002-6350-7728
Krammer, BarbaraUniversity of Applied Sciences Upper Austria, Franz-Fritsch-Straße 11, 4600, Wels, AustriaUNSPECIFIED
Hüsing, NicolaNicola.Huesing (at) sbg.ac.atUNSPECIFIED
Date:23 April 2019
Journal or Publication Title:ACS Applied Materials and Interfaces
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Publisher:American Chemical society (ACS)
ISSN:1944-8244
Status:Published
Keywords:silica, silk fibroin, hybrid aerogel, sol-gel, bone tissue engineering
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Transport
HGF - Program Themes:Road Transport
DLR - Research area:Transport
DLR - Program:V ST Straßenverkehr
DLR - Research theme (Project):V - NGC Fahrzeugstruktur II
Location: Köln-Porz
Institutes and Institutions:Institute of Materials Research > Aerogele
Deposited By: Milow, Dr. Barbara
Deposited On:19 Jan 2021 07:22
Last Modified:19 Jan 2021 07:22

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