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Multimicrobial Kombucha Culture Tolerates Mars-like Conditions Simulated on Low Earth Orbit

Podolich, Olga and Kukharenko, Olga and Haidak, Andriy and Zaets, Iryna and Zaika, Leonid and Storozhuk, Olha and Palchikovska, Larysa and Orlovska, Iryna and Reva, Oleg and Borisova, Tatiana and Khirunenko, Ludmila and Sosnin, Mikhail and Rabbow, Elke and Kravchenko, Volodymyr and Skoryk, Mykola and Kremenskoy, Maksym and Demets, Rene and Olsson-Francis, Karen and Kozyrovska, Natalia and de Vera, Jean-Pierre (2019) Multimicrobial Kombucha Culture Tolerates Mars-like Conditions Simulated on Low Earth Orbit. Astrobiology, 19 (2), pp. 183-196. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2017.1746 ISSN 1531-1074

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Official URL: http://dx.doi.org/10.1089/ast.2017.1746

Abstract

A kombucha multimicrobial culture (KMC) was exposed to simulated Mars-like conditions in low Earth orbit (LEO). The study was part of the BIOlogy and Mars EXperiment (BIOMEX), which was accommodated in the European Space Agency’s EXPOSE-R2 facility, outside the International Space Station. The aim of the study was to investigate the capability of a KMC microecosystem to survive simulated Mars-like conditions in LEO. During the 18-month exposure period, desiccated KMC samples, represented by living cellulose-based films, were subjected to simulated anoxic Mars-like conditions and ultraviolet (UV) radiation, as prevalent at the surface of present-day Mars. Postexposure analysis demonstrated that growth of both the bacterial and yeast members of the KMC community was observed after 60 days of incubation; whereas growth was detected after 2 days in the initial KMC. The KMC that was exposed to extraterrestrial UV radiation showed degradation of DNA, alteration in the composition and structure of the cellular membranes, and an inhibition of cellulose synthesis. In the ‘‘space dark control’’ (exposed to LEO conditions without the UV radiation), the diversity of the microorganisms that survived in the biofilm was reduced compared with the ground-based controls. This was accompanied by structural dissimilarities in the extracellular membrane vesicles. After a series of subculturing, the revived communities restored partially their structure and associated activities.

Item URL in elib:https://elib.dlr.de/126588/
Document Type:Article
Title:Multimicrobial Kombucha Culture Tolerates Mars-like Conditions Simulated on Low Earth Orbit
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Podolich, OlgaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Kukharenko, OlgaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Haidak, AndriyInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Zaets, IrynaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Zaika, LeonidInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Storozhuk, OlhaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Palchikovska, LarysaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Orlovska, IrynaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Reva, OlegDepartment of Biochemistry, Genetics and Microbiology, Centre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, South Africa.UNSPECIFIED
Borisova, TatianaPalladin Institute of Biochemistry of NASU, Kyiv, Ukraine.UNSPECIFIED
Khirunenko, LudmilaInstitute of Physics of NASU, Kyiv, UkraineUNSPECIFIED
Sosnin, MikhailInstitute of Physics of NASU, Kyiv, UkraineUNSPECIFIED
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Kravchenko, VolodymyrNanoMedTech LLC, Kyiv, Ukraine.UNSPECIFIED
Skoryk, MykolaNanoMedTech LLC, Kyiv, Ukraine.UNSPECIFIED
Kremenskoy, MaksymNanoMedTech LLC, Kyiv, Ukraine.UNSPECIFIED
Demets, ReneESA/ESTEC, Noordwijk, The Netherlands.UNSPECIFIED
Olsson-Francis, KarenSchool of Environment, Earth and Ecosystem Sciences, The Open University, Milton Keynes, United KingdomUNSPECIFIED
Kozyrovska, NataliaInstitute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
de Vera, Jean-PierreInstitute of Planetary Research, Management and Infrastructure, Research Group Astrobiology Laboratories, DLR, Berlin, Germanyhttps://orcid.org/0000-0002-9530-5821
Date:11 February 2019
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1089/ast.2017.1746
Page Range:pp. 183-196
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Kombucha multimicrobial culture, Survivability, Biofilm, Extracellular membrane vesicles.
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie, R - Vorhaben Planetary Evolution and Life
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Planetary Research > Leitungsbereich PF
Deposited By: Kopp, Kerstin
Deposited On:15 Mar 2019 09:37
Last Modified:06 Sep 2019 15:23

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