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Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit

Billi, Daniela and Staibano, Clelia and Verseux, Cyprien and Fagliarone, Claudia and Mosca, Claudia and Baque, Mickael and Rabbow, Elke and Rettberg, Petra (2019) Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit. Astrobiology, 19 (8), pp. 1008-1017. Mary Ann Liebert Inc.. doi: 10.1089/ast.2018.1900. ISSN 1531-1074.

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2018.1900

Abstract

Chroococcidiopsis were exposed to low Earth conditions by using the EXPOSE-R2 facility outside the International Space Station. During the space mission, samples in Tray 1 (space vacuum and solar radiation, from λ ≈ 110 nm) and Tray 2 (Mars-like UV flux, λ > 200 nm and Mars-like atmosphere) received total UV (200–400 nm) fluences of about 4.58 × 102 kJ/m² and 4.92 × 102 kJ/m², respectively, and 0.5 Gy of cosmic ionizing radiation. Postflight analyses were performed on 2.5-year-old samples due to the space mission duration, from launch to sample return to the lab. The occurrence of survivors was determined by evaluating cell division upon rehydration and damage to the genome and photosynthetic apparatus by polymerase chain reaction–stop assays and confocal laser scanning microscopy. Biofilms recovered better than their planktonic counterparts, accumulating less damage not only when exposed to UV radiation under space and Mars-like conditions but also when exposed in dark conditions to low Earth conditions and laboratory control conditions. This suggests that, despite the shielding provided by top-cell layers being sufficient for a certain degree of survival of the multilayered planktonic samples, the enhanced survival of biofilms was due to the presence of abundant extracellular polymeric substances and to additional features acquired upon drying.

Item URL in elib:https://elib.dlr.de/126585/
Document Type:Article
Additional Information:Bisher nur online erschienen.
Title:Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Billi, DanielaUniversity of Rome Tor Vergata, Department of Biology, Rome, Italy.UNSPECIFIED
Staibano, CleliaUniversity of Rome Tor Vergata, Department of Biology, Rome, Italy.UNSPECIFIED
Verseux, CyprienUniversity of Rome Tor Vergata, Department of Biology, Rome, Italy.UNSPECIFIED
Fagliarone, ClaudiaUniversity of Rome Tor Vergata, Department of Biology, Rome, Italy.UNSPECIFIED
Mosca, ClaudiaUniversity of Rome Tor Vergata, Department of Biology, Rome, Italy.UNSPECIFIED
Baque, MickaelMickael.Baque (at) dlr.dehttps://orcid.org/0000-0002-6696-6030
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Date:11 February 2019
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1089/ast.2018.1900
Page Range:pp. 1008-1017
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Cyanobacteria, Mars-like, Space, Biofilms, Planktonic, EXPOSE
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Space Exploration
DLR - Research theme (Project):R - Vorhaben Planetary Evolution and Life (old), R - Vorhaben Strahlenbiologie (old)
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:14 Mar 2019 07:47
Last Modified:06 Sep 2019 15:23

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