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Functional brainstem imaging reveals brainstem nuclei governing human baroreflex function

Gerlach, D.A. and Manuel, J. and Hoff, A. and Kronsbein, H. and Hoffmann, F. and Heusser, K. and Beissner, F. and Tank, J. (2018) Functional brainstem imaging reveals brainstem nuclei governing human baroreflex function. In: CLINICAL AUTONOMIC RESEARCH, pp. 453-505. 29TH INTERNATIONAL SYMPOSIUM ON THE AUTONOMIC NERVOUS SYSTEM, 24.-27. Oktober 2018, Newport Beach Marriott; Newport Beach, California. DOI: 10.1007/s10286-018-0565-4

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Official URL: https://link.springer.com/article/10.1007%2Fs10286-018-0565-4

Abstract

Introduction: Brainstem nuclei mediate baroreflex adjustments in efferent sympathetic and parasympathetic traffic. Yet, human brainstem physiology is poorly understood given the lack of suitable methodology. We developed a novel approach combining pharmacological testing, beat-by-beat cardiovascular monitoring, and high-resolution functional magnetic resonance imaging (fMRI) to assess human baroreflex regulation at the level of the brainstem. Methods: In 10 healthy men (29.7 ± 6.9 years; 80.1 ± 9.9 kg), we monitored continuous finger arterial blood pressure and ECG using customized hardware during multiband fMRI brain acquisitions. We applied repeated intravenous phenylephrine (PHE, 25 and 75 lg, n = 8) and nitroprusside (NTP 25 and 75 lg, n = 8) boluses using a remote controlled injector. Brainstem and hypothalamus fMRI Images were analyzed to identify brainstem nuclei involved in baroreflexmediated blood pressure control using tensorial masked Independent Component Analysis (mICA). BP changes were correlated with the time-courses of blood-oxygen-level dependent (BOLD) signals by mixed-effects general linear model. Results: Pharmacological testing yielded reliable baroreflex sensitivity measurements in the MRI scanner. fMRI combined with the measurement of baroreflex-mediated changes in heart rate and blood pressure revealed nuclei regulated through baroreflex input. The strongest correlations between BOLD time-course and BP was observed in the left solitary tract (NTP: deactivation P \0.001; z-score: 6.9), the caudal ventrolateral medulla (NTP: activation, right side: P = 0.0012; z-score: 4.11, left side: P\0.001; z-score: 5.05), the rostral ventrolateral medulla (NTP: activation, right side P\ 0.001; z-score: 7.25) and in the left paraventricular nucleus (PHE: activation P \0.001; z-score: 9.00; NTP: activation P\ 0.001; z-score: 7.11). P-values were Bonferroni corrected for 60 independent components. Conclusion: We developed novel approach testing baroreflex regulation at the level of the brainstem in humans. The methodology identified baroreflex-mediated activation and deactivation Patterns consistent with previous investigations in animal models. The methodology can be applied to elucidate human physiology and mechanisms of autonomic cardiovascular disease.

Item URL in elib:https://elib.dlr.de/126176/
Document Type:Conference or Workshop Item (Speech)
Title:Functional brainstem imaging reveals brainstem nuclei governing human baroreflex function
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Gerlach, D.A.darius.gerlach (at) dlr.dehttps://orcid.org/0000-0001-7044-6065
Manuel, J.UNSPECIFIEDUNSPECIFIED
Hoff, A.alex.hoff (at) dlr.deUNSPECIFIED
Kronsbein, H.UNSPECIFIEDUNSPECIFIED
Hoffmann, F.fabian.hoffmann (at) dlr.deUNSPECIFIED
Heusser, K.Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Beissner, F.UNSPECIFIEDUNSPECIFIED
Tank, J.Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Date:October 2018
Journal or Publication Title:CLINICAL AUTONOMIC RESEARCH
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
DOI :10.1007/s10286-018-0565-4
Page Range:pp. 453-505
Status:Published
Keywords:Brainstem Imaging; brainstem nuclei; baroreflex function
Event Title:29TH INTERNATIONAL SYMPOSIUM ON THE AUTONOMIC NERVOUS SYSTEM
Event Location:Newport Beach Marriott; Newport Beach, California
Event Type:international Conference
Event Dates:24.-27. Oktober 2018
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Systemphysiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Cardiovascular Medicine in Aerospace
Deposited By: Schrage, Larissa
Deposited On:24 Jan 2019 11:57
Last Modified:31 Jul 2019 20:24

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