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Restart capability of resting-states of Euglena gracilis after 9 months of dormancy: preparation for autonomous space flight experiments

Strauch, Sebastian M. and Becker, Ina and Pölloth, Laura and Richter, Peter R. and Haag, Ferdinand W.M. and Hauslage, Jens and Lebert, Michael (2018) Restart capability of resting-states of Euglena gracilis after 9 months of dormancy: preparation for autonomous space flight experiments. International Journal of Astrobiology, 17 (2), pp. 101-111. Cambridge University Press. DOI: 10.1017/S1473550417000131 ISSN 1473-5504

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Official URL: https://www.cambridge.org/core/journals/international-journal-of-astrobiology/article/restart-capability-of-restingstates-of-euglena-gracilis-after-9-months-of-dormancy-preparation-for-autonomous-space-flight-experiments/1564B49449577C9EE43AD9FA120002BA

Abstract

Dormant states of organisms are easier to store than the living state because they tolerate larger variations in temperature, light, storage space etc., making them attractive for laboratory culture stocks and also for experiments under special circumstances, especially space flight experiments. Like several other organisms, Euglena gracilis is capable of forming desiccation tolerant resting states in order to survive periods of unfavourable environmental conditions. In earlier experiments it was found that dormant Euglena cells must not become completely desiccated. Some residual moisture is required to ensure recovery of the resting states. To analyse the water demand in recovery of Euglena resting states, cells were transferred to a defined amount of cotton wool (0.5 g). Subsequently different volumes of medium (1, 2, 3, 4, 5, 8, 10 and 20 ml) were added in order to supply humidity; a control was set up without additional liquid. Samples were sealed in transparent 50 ml falcon tubes and stored for 9 months under three different conditions: - constant low light conditions in a culture chamber at 20°C, - in a black box, illuminated with short light emitting diode-light pulses provided by joule thieves and - in darkness in a black box. After 9 months, cells were transferred to fresh medium and cell number, photosynthetic efficiency and movement behavior was monitored over 3 weeks. It was found that cells recovered under all conditions except in the control, where no medium was supplied. Transcription levels of 21 genes were analysed with a Multiplexpolymerase chain reaction. One hour after rehydration five of these genes were found to be up-regulated: ubiquitin, heat shock proteins HSP70, HSP90, the calcium-sensor protein frequenin and a distinct protein kinase, which is involved in gravitaxis. The results indicate a transient general stress response of the cells.

Item URL in elib:https://elib.dlr.de/121662/
Document Type:Article
Title:Restart capability of resting-states of Euglena gracilis after 9 months of dormancy: preparation for autonomous space flight experiments
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Strauch, Sebastian M.Friedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0433-5874
Becker, InaDLR Space Life Institute for Aerospace Medicine, Gravitational Biology, Cologne, Germany, and Friedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, GermanyUNSPECIFIED
Pölloth, LauraFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, GermanyUNSPECIFIED
Richter, Peter R.Department of Biology, Cell Biology Division, Friedrich Alexander University, Erlangen, Erlangen, GermanyUNSPECIFIED
Haag, Ferdinand W.M.Friedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0003-2146-793X
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Lebert, MichaelFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0448-8117
Date:2018
Journal or Publication Title:International Journal of Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:17
DOI :10.1017/S1473550417000131
Page Range:pp. 101-111
Publisher:Cambridge University Press
ISSN:1473-5504
Status:Published
Keywords:dormancy, Euglena gracilis, frequenin, HSP70, HSP90, Multiplex-PCR, PKA, recovery, resting states, ubiquitin
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung, R - Project eu:cropis
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:20 Sep 2018 17:51
Last Modified:06 Sep 2019 15:15

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