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Pathway Analysis Hints Towards Benefcial Effects of Long-Term Vibration on Human Chondrocytes

Lützenberg, Roland and Solano, Kendrick and Buken, Christoph and Sahana, Jayashree and Riwaldt, Stefan and Kopp, Sascha and Krüger, Marcus and Schulz, Herbert and Saar, Kathrin and Huebner, Norbert and Hemmersbach, Ruth and Bauer, Johann and Infanger, Manfred and Grimm, Daniela and Wehland, Markus (2018) Pathway Analysis Hints Towards Benefcial Effects of Long-Term Vibration on Human Chondrocytes. Cellular Physiology and Biochemistry (47), pp. 1729-1741. S. Karger AG. DOI: 10.1159/00049100 ISSN 1015-8987

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Official URL: https://www.karger.com/Article/FullText/491006

Abstract

Background/Aims: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes. Methods: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profle which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis. Results: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were signifcantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecifc stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM. Conclusions: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes.

Item URL in elib:https://elib.dlr.de/121565/
Document Type:Article
Title:Pathway Analysis Hints Towards Benefcial Effects of Long-Term Vibration on Human Chondrocytes
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Lützenberg, Rolandotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Solano, Kendrickotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Buken, Christophotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Sahana, JayashreeAarhus University, Institute of Biomedicine, Pharmacology, Aarhus, DenmarkUNSPECIFIED
Riwaldt, StefanAarhus University, Institute of Biomedicine, Pharmacology, Aarhus, DenmarkUNSPECIFIED
Kopp, Saschaotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Krüger, Marcusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Schulz, HerbertUniversity of Cologne, Cologne Center for Genomics, Cologne, GermanyUNSPECIFIED
Saar, KathrinMax-Delbrück Center for Molecular Medicine, Experimental Genetics of Cardiovascular Diseases, Berlin-Buch, GermanyUNSPECIFIED
Huebner, NorbertMax-Delbrück Center for Molecular Medicine, Experimental Genetics of Cardiovascular Diseases, Berlin-Buch, GermanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Infanger, Manfredotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Grimm, DanielaOtto-von-Guericke-University-Magdeburg, Gravitational Biology and Translational Regenerativev Medicine, Faculty of Medicine and Mechanical Engineering, Magdeburg, GermanyUNSPECIFIED
Wehland, Markusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Date:28 June 2018
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1159/00049100
Page Range:pp. 1729-1741
Publisher:S. Karger AG
ISSN:1015-8987
Status:Published
Keywords:Chondrocytes, Vibration, Cytoskeleton, Extracellular matrix proteins, Gene expression, Pathway analysis
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:06 Sep 2018 18:06
Last Modified:23 Jan 2019 05:02

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