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Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy

Fuchs, Felix M. and Holland, Gudrun and Moeller, Ralf and Laue, Michael (2018) Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy. Journal of Microbiological Methods, 152, pp. 165-172. Elsevier. DOI: 10.1016/j.mimet.2018.08.005 ISSN 0167-7012

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.mimet.2018.08.005

Abstract

Biofilms have been intensively investigated over the past decades. Bacillus subtilis is able to form highly structured colony biofilms, and as one of the most studied Gram-positive model organisms, has helped to decipher the complex genetic regulation of biofilms. Several methods have been developed to analyze the architecture of biofilms. In this paper, we describe a method which allows the analysis of the internal structures of biofilms by scanning electron microscopy (SEM). The method uses a modified freeze-fracturing of chemically fixed biofilm to generate defined, well-preserved fractures at millimeter-scale which allows to analyze systematically the internal biofilm structure from macro- to nano-scale.

Item URL in elib:https://elib.dlr.de/121492/
Document Type:Article
Title:Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fuchs, Felix M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655
Holland, GudrunRobert Koch Institute, Advanced Light and Electron Microscopy (ZBS 4), Berlin, GermanyUNSPECIFIED
Moeller, RalfRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Laue, MichaelRobert Koch Institute, Advanced Light and Electron Microscopy (ZBS 4), Berlin, GermanyUNSPECIFIED
Date:17 August 2018
Journal or Publication Title:Journal of Microbiological Methods
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:152
DOI :10.1016/j.mimet.2018.08.005
Page Range:pp. 165-172
Publisher:Elsevier
ISSN:0167-7012
Status:Published
Keywords:B. subtilis; Biofilm; Cross-section; SEM; Filter
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:12 Sep 2018 09:24
Last Modified:12 Sep 2018 09:24

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