Fuchs, Felix M. and Holland, Gudrun and Moeller, Ralf and Laue, Michael (2018) Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy. Journal of Microbiological Methods, 152, pp. 165-172. Elsevier. doi: 10.1016/j.mimet.2018.08.005. ISSN 0167-7012.
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.mimet.2018.08.005
Abstract
Biofilms have been intensively investigated over the past decades. Bacillus subtilis is able to form highly structured colony biofilms, and as one of the most studied Gram-positive model organisms, has helped to decipher the complex genetic regulation of biofilms. Several methods have been developed to analyze the architecture of biofilms. In this paper, we describe a method which allows the analysis of the internal structures of biofilms by scanning electron microscopy (SEM). The method uses a modified freeze-fracturing of chemically fixed biofilm to generate defined, well-preserved fractures at millimeter-scale which allows to analyze systematically the internal biofilm structure from macro- to nano-scale.
Item URL in elib: | https://elib.dlr.de/121492/ | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Document Type: | Article | |||||||||||||||
Title: | Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy | |||||||||||||||
Authors: |
| |||||||||||||||
Date: | 17 August 2018 | |||||||||||||||
Journal or Publication Title: | Journal of Microbiological Methods | |||||||||||||||
Refereed publication: | Yes | |||||||||||||||
Open Access: | No | |||||||||||||||
Gold Open Access: | No | |||||||||||||||
In SCOPUS: | Yes | |||||||||||||||
In ISI Web of Science: | Yes | |||||||||||||||
Volume: | 152 | |||||||||||||||
DOI: | 10.1016/j.mimet.2018.08.005 | |||||||||||||||
Page Range: | pp. 165-172 | |||||||||||||||
Publisher: | Elsevier | |||||||||||||||
ISSN: | 0167-7012 | |||||||||||||||
Status: | Published | |||||||||||||||
Keywords: | B. subtilis; Biofilm; Cross-section; SEM; Filter | |||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | |||||||||||||||
HGF - Program: | Space | |||||||||||||||
HGF - Program Themes: | Research under Space Conditions | |||||||||||||||
DLR - Research area: | Raumfahrt | |||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | |||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Strahlenbiologie (old) | |||||||||||||||
Location: | Köln-Porz | |||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Radiation Biology | |||||||||||||||
Deposited By: | Kopp, Kerstin | |||||||||||||||
Deposited On: | 12 Sep 2018 09:24 | |||||||||||||||
Last Modified: | 12 Sep 2018 09:24 |
Repository Staff Only: item control page