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Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400 nm Blue Light

Djouiai, Bahar and Thwaite, Joanne E. and Laws, Thomas R. and Commichau, Fabian M. and Setlow, Barbara and Setlow, Peter and Moeller, Ralf (2018) Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400 nm Blue Light. Applied and Environmental Microbiology, 84 (19), AEM.01604-18. American Society for Microbiology. DOI: 10.1128/AEM.01604-18 ISSN 0099-2240

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Official URL: http://dx.doi.org/10.1128/AEM.01604-18

Abstract

The high intrinsic decontamination resistance of Firmicutes spores, is important medically (disease) and commercially (food spoilage). Effective methods of spore eradication would be of considerable interest in the health care and medical products industries; particularly if the decontamination method effectively killed spores while remaining benign to both humans and sensitive equipment. Intense blue light at ∼ 400 nm is one such treatment that has drawn significant interest. This work has determined the resistance of spores to blue light in an extensive panel of Bacillus subtilis strains, including wild-type strains and mutants that: i) lack protective components such as the spore coat and its pigment (s) or the DNA protective α/β-type small acid-soluble spore proteins (SASP); ii) have an elevated spore core water content; or iii) lack enzymes involved in DNA repair, including homologous recombination and non-homologous end joining (HR and NHEJ), apurinic/apyrimidinic endonucleases, nucleotide- and base excision repair (NER & BER), translesion synthesis (TLS) by Y-family DNA polymerases and spore photoproduct (SP) removal by SP lyase (SPL). The most important factors in spore blue light resistance were determined to be: spore coats/pigmentation, α/β-type SASP, NER, BER TLS and SP repair. A major conclusion from this work is that blue light kills spores by DNA-damage, and the results in this work indicate at least some of the specific DNA-damage. It appears that high-intensity blue light could be a significant addition to the agents used to kill bacterial spores in applied settings.

Item URL in elib:https://elib.dlr.de/121402/
Document Type:Article
Title:Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400 nm Blue Light
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Djouiai, BaharRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Thwaite, Joanne E.Defence Science and Technology Laboratory, Chemical, Biological and Radiological Division, Salisbury, United KingdomUNSPECIFIED
Laws, Thomas R.Chemical, Biological and Radiological Division, Dstl, Porton Down, Salisbury, Wiltshire, United KingdomUNSPECIFIED
Commichau, Fabian M.Department of General Microbiology, University of Göttingen, Grisebachstrasse 8, D-37077 Göttingen, GermanyUNSPECIFIED
Setlow, BarbaraDepartment of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA.UNSPECIFIED
Setlow, PeterDepartment of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA.UNSPECIFIED
Moeller, RalfRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:27 July 2018
Journal or Publication Title:Applied and Environmental Microbiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:84
DOI :10.1128/AEM.01604-18
Page Range:AEM.01604-18
Publisher:American Society for Microbiology
ISSN:0099-2240
Status:Published
Keywords:spore inactivation, Bacillus subtilis spore resistance, 400 nm blue light
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:27 Nov 2018 08:54
Last Modified:06 Sep 2019 15:19

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