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Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity

Acharya, Aviseka and Brungs, Sonja and Henry, Margit and Rotshteyn, Tamara and Yaduvanshi, Nirmala Singh and Wegener, Lucia and Jentzsch, Simon and Hescheler, Jürgen and Hemmersbach, Ruth and Boeuf, Helene and Sachinidis, Apapios (2018) Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity. Stem Cells and Development, 27 (12), pp. 838-847. Mary Ann Liebert. DOI: 10.1089/scd.2017.0294 ISSN 1547-3287

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Official URL: https://www.liebertpub.com/doi/10.1089/scd.2017.0294

Abstract

Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of short-term altered gravity on embryonic development processes, we exposed mouse embryonic stem cells (mESCs) to phases of hypergravity and microgravity and studied the differentiation potential of the cells using wide-genome microarray analysis. During the 64th European Space Agency’s parabolic flight campaign, mESCs were exposed to 31 parabolas. Each parabola comprised phases lasting 22 s of hypergravity, microgravity, and a repeat of hypergravity. On different parabolas, RNA was isolated for microarray analysis. After exposure to 31 parabolas, mESCs (P31 mESCs) were further differentiated under normal gravity (1 g) conditions for 12 days, producing P31 12-day embryoid bodies (EBs). After analysis of the microarrays, the differentially expressed genes were analyzed using different bioinformatic tools to identify developmental and nondevelopmental biological processes affected by conditions on the parabolic flight experiment. Our results demonstrated that several genes belonging to GOs associated with cell cycle and proliferation were downregulated in undifferentiated mESCs exposed to gravity changes. However, several genes belonging to developmental processes, such as vasculature development, kidney development, skin development, and to the TGF-b signaling pathway, were upregulated. Interestingly, similar enriched and suppressed GOs were obtained in P31 12-day EBs compared with ground control 12-day EBs. Our results show that undifferentiated mESCs exposed to alternate hypergravity and microgravity phases expressed several genes associated with developmental/ differentiation and cell cycle processes, suggesting a transition from the undifferentiated pluripotent to a more differentiated stage of mESCs.

Item URL in elib:https://elib.dlr.de/121273/
Document Type:Article
Title:Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Acharya, AvisekaUniversity of Cologne, Institute of Neurophysiology, Center of Physiology and Pathophysiology, Cologne, GermanyUNSPECIFIED
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Henry, MargitUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Rotshteyn, TamaraUniversity of Cologne, Institute of Neurophysiology, Center of Physiology and Pathophysiology, Cologne, GermanyUNSPECIFIED
Yaduvanshi, Nirmala SinghUniversity of Cologne, Institute of Neurophysiology, Center of Physiology and Pathophysiology, Cologne, GermanyUNSPECIFIED
Wegener, Luciagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Jentzsch, Simongerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hescheler, JürgenUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Boeuf, HeleneINSERM-U1026, BioTis, University of Bordeaux, Bordeaux, FranceUNSPECIFIED
Sachinidis, ApapiosUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Date:13 March 2018
Journal or Publication Title:Stem Cells and Development
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:27
DOI :10.1089/scd.2017.0294
Page Range:pp. 838-847
Publisher:Mary Ann Liebert
ISSN:1547-3287
Status:Published
Keywords:microgravity, hypergravity, embryonic stem cells, differentiation, transcriptomics, parabolic flight
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:14 Aug 2018 10:00
Last Modified:23 Feb 2019 00:22

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