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Design concepts in absorbance optical systems for analytical ultracentrifugation

Pearson, Joseph and Hofstetter, Marcel and Dekorsy, Thomas and Totzeck, Michael and Cölfen, Helmut (2018) Design concepts in absorbance optical systems for analytical ultracentrifugation. The Analyst. Royal Society of Chemistry. DOI: 10.1039/C8AN00706C ISSN 0003-2654

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Official URL: http://pubs.rsc.org/en/content/articlelanding/2018/an/c8an00706c#!divAbstract

Abstract

Analytical ultracentrifugation is a powerful technique for analyzing particles in solution, and has proved valuable for a wide range of applications in chemistry, biochemistry and material sciences for many years. The field is presently seeing a resurgence of instrument development from commercial and academic groups. To date, no modern optical modeling techniques have ever been applied to the basic imaging properties of the optical system in analytical ultracentrifugation. In this manuscript we provide a contextual framework for the application of such techniques, including an overview of the essential optical principles. The existing commercial and open source detection systems are evaluated for imaging performance, highlighting the limitations of chromatic aberration for broadband acquisitions. These results are the inspiration for a new mirror-based design, free of chromatic aberration. Our findings present a path forward for continued development in imaging and detector technology, where improved data quality will now push the limits of detection and resolution of analytical ultracentrifugation for years to come.

Item URL in elib:https://elib.dlr.de/120990/
Document Type:Article
Title:Design concepts in absorbance optical systems for analytical ultracentrifugation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Pearson, JosephUniversity of KonstanzUNSPECIFIED
Hofstetter, MarcelUniversity of KonstanzUNSPECIFIED
Dekorsy, ThomasInstitute of Technical Physics, DLRhttps://orcid.org/0000-0003-2257-2854
Totzeck, MichaelCarl Zeiss AGUNSPECIFIED
Cölfen, HelmutUniversity of KonstanzUNSPECIFIED
Date:28 June 2018
Journal or Publication Title:The Analyst
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1039/C8AN00706C
Publisher:Royal Society of Chemistry
ISSN:0003-2654
Status:Published
Keywords:ultracentrifugation, ray traycing, technical optics
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Aeronautics
HGF - Program Themes:fixed-wing aircraft
DLR - Research area:Aeronautics
DLR - Program:L AR - Aircraft Research
DLR - Research theme (Project):L - Laser Research and Technology
Location: Stuttgart
Institutes and Institutions:Institute of Technical Physics
Deposited By: Dekorsy, Prof. Dr. Thomas
Deposited On:16 Jul 2018 11:25
Last Modified:16 Jul 2018 11:25

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