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Validation of Microgravity Simulators (Random Positioning Machine and Clinostat) Using Cellular Bioassays

Brungs, Sonja and Hauslage, Jens and Cevik, Volkan and Waßer, Kai and Hemmersbach, Ruth (2018) Validation of Microgravity Simulators (Random Positioning Machine and Clinostat) Using Cellular Bioassays. International Society für Gravitational Physiology & European Space Agency Life Sciences Meeting 2018, 18. – 22 Juni 2018, Noordwijk, The Netherlands.

Full text not available from this repository.

Abstract

In the ESA ground-based facility program, clinostats and Random Positioning Machines (RPM) are readily available for external scientists to apply simulated microgravity, and results from experiments in cell biology have been recently published. The results clearly demonstrate that device-specific operational modes have to be carefully considered and comprehensively discussed with respect to the induction of shear forces by the various simulators, which have a considerable impact on the quality of (simulated) microgravity. In order to directly visualize potential non-gravitational cellular responses during simulator operation, we exposed the dinoflagellate Pyrocystis noctiluca as a highly sensitive reporter organism, which responds to shear stress with detectable bioluminescence emission, to clinorotation and random positioning. Rotation around two axes with random velocities and directions (RPM mode) revealed significantly greater mechanical stress responses compared with constant and fast rotation around one axis (clinostat). Another bioluminescence assay using a well-studied immune cell (macrophage) system also showed that the oxidative burst response during RPM-exposure differs significantly from that observed during clinorotation. We conclude that fast one axis clinorotation should be the preferred method for simulation of microgravity in experiments using cells.

Item URL in elib:https://elib.dlr.de/120561/
Document Type:Conference or Workshop Item (Speech)
Title:Validation of Microgravity Simulators (Random Positioning Machine and Clinostat) Using Cellular Bioassays
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Cevik, Volkangerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Waßer, KaiGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, GermanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Date:18 June 2018
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Microgravity simulators, Random Positioning Machine, Clinostat, Bioassays
Event Title:International Society für Gravitational Physiology & European Space Agency Life Sciences Meeting 2018
Event Location:Noordwijk, The Netherlands
Event Type:international Conference
Event Dates:18. – 22 Juni 2018
Organizer:International Society of Gravitational Physiology (ISGP) and European Space Agency (ESA)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:14 Aug 2018 09:58
Last Modified:14 Aug 2018 09:58

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