Brungs, Sonja und Hauslage, Jens und Cevik, Volkan und Waßer, Kai und Hemmersbach, Ruth (2018) Validation of Microgravity Simulators (Random Positioning Machine and Clinostat) Using Cellular Bioassays. International Society für Gravitational Physiology & European Space Agency Life Sciences Meeting 2018, 2018-06-18 - 2018-06-22, Noordwijk, The Netherlands.
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Kurzfassung
In the ESA ground-based facility program, clinostats and Random Positioning Machines (RPM) are readily available for external scientists to apply simulated microgravity, and results from experiments in cell biology have been recently published. The results clearly demonstrate that device-specific operational modes have to be carefully considered and comprehensively discussed with respect to the induction of shear forces by the various simulators, which have a considerable impact on the quality of (simulated) microgravity. In order to directly visualize potential non-gravitational cellular responses during simulator operation, we exposed the dinoflagellate Pyrocystis noctiluca as a highly sensitive reporter organism, which responds to shear stress with detectable bioluminescence emission, to clinorotation and random positioning. Rotation around two axes with random velocities and directions (RPM mode) revealed significantly greater mechanical stress responses compared with constant and fast rotation around one axis (clinostat). Another bioluminescence assay using a well-studied immune cell (macrophage) system also showed that the oxidative burst response during RPM-exposure differs significantly from that observed during clinorotation. We conclude that fast one axis clinorotation should be the preferred method for simulation of microgravity in experiments using cells.
elib-URL des Eintrags: | https://elib.dlr.de/120561/ | ||||||||||||||||||||||||
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Dokumentart: | Konferenzbeitrag (Vortrag) | ||||||||||||||||||||||||
Titel: | Validation of Microgravity Simulators (Random Positioning Machine and Clinostat) Using Cellular Bioassays | ||||||||||||||||||||||||
Autoren: |
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Datum: | 18 Juni 2018 | ||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||
Open Access: | Nein | ||||||||||||||||||||||||
Gold Open Access: | Nein | ||||||||||||||||||||||||
In SCOPUS: | Nein | ||||||||||||||||||||||||
In ISI Web of Science: | Nein | ||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||
Stichwörter: | Microgravity simulators, Random Positioning Machine, Clinostat, Bioassays | ||||||||||||||||||||||||
Veranstaltungstitel: | International Society für Gravitational Physiology & European Space Agency Life Sciences Meeting 2018 | ||||||||||||||||||||||||
Veranstaltungsort: | Noordwijk, The Netherlands | ||||||||||||||||||||||||
Veranstaltungsart: | internationale Konferenz | ||||||||||||||||||||||||
Veranstaltungsbeginn: | 18 Juni 2018 | ||||||||||||||||||||||||
Veranstaltungsende: | 22 Juni 2018 | ||||||||||||||||||||||||
Veranstalter : | International Society of Gravitational Physiology (ISGP) and European Space Agency (ESA) | ||||||||||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (alt), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt) | ||||||||||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie | ||||||||||||||||||||||||
Hinterlegt von: | Duwe, Helmut | ||||||||||||||||||||||||
Hinterlegt am: | 14 Aug 2018 09:58 | ||||||||||||||||||||||||
Letzte Änderung: | 24 Apr 2024 20:24 |
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