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Hypergravitation selectively augments neuronal in vitro differentiation

Brungs, Sonja and Liemersdorf, Christian and Lichterfeld, Yannick and Frett, Timo and Anken, Ralf and Jordan, Jens and Hemmersbach, Ruth (2018) Hypergravitation selectively augments neuronal in vitro differentiation. In: FASEB JOURNAL. Experimental Biology 2018, 21. - 25. April 2018, San Diego, USA.

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Disturbed neuronal connectivity is the ultimate cause of disability in individuals with neurological disease including spinal cord injury, head trauma, and stroke. Functional neurological recovery is limited through an unfavorable balance between neuronal regrowth and glia scar formation. Neuronal growth requires dynamic cytoskeletal protein rearrangements. Because hypergravity stabilizes microtubules while de-stabilizing actin filaments, we hypothesized that experimental hypergravity would shift the balance between neuronal and astroglial growth in vitro. We exposed murine primary hippocampal neurons during different developmental stages to 2g using the DLR hypergravity platform. The platform unlike commercial laboratory centrifuges models physiological hypergravity and allows for cell cultivation and live-cell imaging. We assessed neuritogenesis, neuronal polarization and maturation processes including synaptogenesis and synaptic integration in mature neural networks. Moreover, we studied primary astrocytes to shed light on their role during glial scar formation at neural lesion sites. Exposure of hippocampal neurons to 24h of 2g hypergravity increased neurite number by 30% and neurite projection length by 20% compared to 1g. At later developmental stages, mature synaptic contacts were formed under hypergravity conditions. In contrast, astrocytes showed decreases in cell spreading and lamellipodial protrusions with hypergravity.We conclude that experimental hypergravity ameliorates neuronal cell growth and synaptic contacts while halting astrocyte spreading and migration in vivo. Given the importance of this balance for neuronal regeneration in human neurological disease, we will now study the underlying mechanisms in more detail using, both, DLR hypergravity and clinostat-induced hypogravity platforms.

Item URL in elib:https://elib.dlr.de/120560/
Document Type:Conference or Workshop Item (Poster)
Additional Information:This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Title:Hypergravitation selectively augments neuronal in vitro differentiation
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Liemersdorf, Christiangravitational biology, institute of aerospace medicine, german aerospace centre (dlr), cologne, germanyhttps://orcid.org/0000-0001-8407-5226
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920
Frett, Timogerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-5572-1177
Anken, Ralfgerman aerospace center (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-7490-6823
Jordan, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Date:21 April 2018
Journal or Publication Title:FASEB JOURNAL
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In ISI Web of Science:Yes
Keywords:Hypergravitation, Neurons, Neuronal differentiation
Event Title:Experimental Biology 2018
Event Location:San Diego, USA
Event Type:international Conference
Event Dates:21. - 25. April 2018
Organizer:American Physiological Society and four other societies
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):Vorhaben: NeuroTox, R - Vorhaben Biowissenschaftliche Exp.-vorbereitung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:03 Jul 2018 13:56
Last Modified:03 Jul 2018 13:56

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