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Hypergravitation selectively augments neuronal in vitro differentiation

Brungs, Sonja und Liemersdorf, Christian und Lichterfeld, Yannick und Frett, Timo und Anken, Ralf und Jordan, Jens und Hemmersbach, Ruth (2018) Hypergravitation selectively augments neuronal in vitro differentiation. In: FASEB JOURNAL. Experimental Biology 2018, 2018-04-21 - 2018-04-25, San Diego, USA.

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Kurzfassung

Disturbed neuronal connectivity is the ultimate cause of disability in individuals with neurological disease including spinal cord injury, head trauma, and stroke. Functional neurological recovery is limited through an unfavorable balance between neuronal regrowth and glia scar formation. Neuronal growth requires dynamic cytoskeletal protein rearrangements. Because hypergravity stabilizes microtubules while de-stabilizing actin filaments, we hypothesized that experimental hypergravity would shift the balance between neuronal and astroglial growth in vitro. We exposed murine primary hippocampal neurons during different developmental stages to 2g using the DLR hypergravity platform. The platform unlike commercial laboratory centrifuges models physiological hypergravity and allows for cell cultivation and live-cell imaging. We assessed neuritogenesis, neuronal polarization and maturation processes including synaptogenesis and synaptic integration in mature neural networks. Moreover, we studied primary astrocytes to shed light on their role during glial scar formation at neural lesion sites. Exposure of hippocampal neurons to 24h of 2g hypergravity increased neurite number by 30% and neurite projection length by 20% compared to 1g. At later developmental stages, mature synaptic contacts were formed under hypergravity conditions. In contrast, astrocytes showed decreases in cell spreading and lamellipodial protrusions with hypergravity.We conclude that experimental hypergravity ameliorates neuronal cell growth and synaptic contacts while halting astrocyte spreading and migration in vivo. Given the importance of this balance for neuronal regeneration in human neurological disease, we will now study the underlying mechanisms in more detail using, both, DLR hypergravity and clinostat-induced hypogravity platforms.

elib-URL des Eintrags:https://elib.dlr.de/120560/
Dokumentart:Konferenzbeitrag (Poster)
Zusätzliche Informationen:This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Titel:Hypergravitation selectively augments neuronal in vitro differentiation
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942NICHT SPEZIFIZIERT
Liemersdorf, Christiangravitational biology, institute of aerospace medicine, german aerospace centre (dlr), cologne, germanyhttps://orcid.org/0000-0001-8407-5226NICHT SPEZIFIZIERT
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920NICHT SPEZIFIZIERT
Frett, Timogerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-5572-1177NICHT SPEZIFIZIERT
Anken, Ralfgerman aerospace center (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-7490-6823NICHT SPEZIFIZIERT
Jordan, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715NICHT SPEZIFIZIERT
Datum:21 April 2018
Erschienen in:FASEB JOURNAL
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Ja
Status:veröffentlicht
Stichwörter:Hypergravitation, Neurons, Neuronal differentiation
Veranstaltungstitel:Experimental Biology 2018
Veranstaltungsort:San Diego, USA
Veranstaltungsart:internationale Konferenz
Veranstaltungsbeginn:21 April 2018
Veranstaltungsende:25 April 2018
Veranstalter :American Physiological Society and four other societies
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):Vorhaben: NeuroTox (alt), R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Hinterlegt von: Duwe, Helmut
Hinterlegt am:03 Jul 2018 13:56
Letzte Änderung:24 Apr 2024 20:24

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