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Bioluminescence systems in environmental biosensors

Erzinger, Gilmar S. and Schmoeller, Francine and Pinto, Luis H. and Américo, Luiz and Hemmersbach, Ruth and Hauslage, Jens and Häder, Donat-Peter (2018) Bioluminescence systems in environmental biosensors. In: Bioassays. Advanced Methods and Applications Elsevier. pp. 241-262. doi: 10.1016/B978-0-12-811861-0.00012-7. ISBN 978-0128118610, 012811861X.

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Official URL: https://www.sciencedirect.com/science/article/pii/B9780128118610000127


This chapter describes the application of potentiometric multisensor systems for determining the toxicity of water samples using the Vibrio fischeri bioluminescence inhibition assay, which is often chosen as the first test in a test battery based on speed and cost consideration. The test protocol is simple and was originally applied to aqueous samples or extracts. Several authors have reported on the bioluminescence assay with V. fischeri as the most sensitive one for a wide range of chemicals as compared with other assays such as growth inhibition in microcrustaceans, physiological and biochemical evaluation of algae and higher animals, and nitrification enzyme inhibition among others. This chapter presents a brief historical summary, and discusses the biochemical aspects which result in the variation of the bioluminescence of V. fischeri. It describes different commercial systems that use this technique as well as the use of genetically modified microorganisms as a means of introducing more sensitive methods using the principles of bioluminescence. These assays show a good correlation with other standard acute toxicity tests and are reported to detect toxicity over a wide range of toxic substances. Dinoflagellates also produce bioluminescence. In these organisms mechanical stimuli induce the light emission, so that they can be employed as bioassays for mechanical stress.

Item URL in elib:https://elib.dlr.de/120559/
Document Type:Book Section
Title:Bioluminescence systems in environmental biosensors
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715UNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000UNSPECIFIED
Häder, Donat-PeterEmeritus from friedrich-alexander-universität erlangen, institut für biologiehttps://orcid.org/0000-0002-4295-5660UNSPECIFIED
Journal or Publication Title:Bioassays. Advanced Methods and Applications
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In ISI Web of Science:No
Page Range:pp. 241-262
ISBN:978-0128118610, 012811861X
Keywords:Bioluminescent organisms, Vibrio fischeri, ToxAlert, Microtox, LUMIStox, Toxicity assays, Effective concentrations, Dinoflagellates, Mechanical stress
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (old), R - Project eu:cropis (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:03 Jul 2018 13:54
Last Modified:03 Jul 2018 13:54

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