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Effects of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Blue-Light Radiation

Djouiai, Bahar (2018) Effects of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Blue-Light Radiation. Master's, Technische Universität Darmstadt.

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Due to the high resistance and potential roles in human disease and food spoilage of spores of Firmicutes, methods for spore eradication are of significant interest for medical products, food- and health care industries and in particular methods that effectively kill spores while remaining relatively benign to humans and sensitive equipment. Intense blue-light at ~ 380 - 450 nm is one such treatment that has drawn significant interest. This work has determined the resistance of spores to blue-light under oxic and anoxic conditions in an extensive panel of Bacillus subtilis strains, including wild-type stains and mutants which: i) lack protective components such as the spore coat and its pigment(s) or the DNA protective α/β-type small acid-soluble spore proteins (SASP); ii) have an elevated spore core water content; or iii) lack enzymes involved in DNA-repair, including homologous recombination and non homologous end joining (HR and NHEJ), apurinic/apyrimidinic endonucleases, nucleotide- and base excision repair (NER & BER), translesion synthesis (TLS) by Y-family DNA polymerases and spore photoproduct (SP) removal by SP lyase (SPL). The most important factors in spore blue-light resistance were determined to be: spore coats/pigmentation, α/β-type SASP, NER, BER, TLS and SP repair. However, repair of DNA double-stand breaks by HR or NHEJ and the spore core water content had only minor roles in spore blue-light resistance. A major conclusion from this work is that blue-light kills spores by DNA damage, and the results in this work indicate at least some of the specific DNA damage. It appears that high intensity blue-light in combination with oxygen could be a significant addition to the agents used to kill bacterial spores in applied settings.

Item URL in elib:https://elib.dlr.de/120259/
Document Type:Thesis (Master's)
Title:Effects of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Blue-Light Radiation
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Djouiai, BaharRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Date:2 May 2018
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In ISI Web of Science:No
Number of Pages:47
Keywords:Bacillus subtilis; spore resistance, blue-light radiation
Institution:Technische Universität Darmstadt
Department:Technical Biology
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:29 Jun 2018 10:14
Last Modified:29 Jun 2018 10:14

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