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Analysis of Statoliths Displacement in Chara Rhizoids for Validating the Microgravity-Simulation Quality of Clinorotation Modes

Krause, Lars and Braun, Markus and Hauslage, Jens and Hemmersbach, Ruth (2018) Analysis of Statoliths Displacement in Chara Rhizoids for Validating the Microgravity-Simulation Quality of Clinorotation Modes. Microgravity Science and Technology, 30 (3), pp. 229-236. Springer. DOI: 10.1007/s12217-017-9580-7 ISSN 0938-0108

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Official URL: https://link.springer.com/article/10.1007/s12217-017-9580-7

Abstract

In single-celled rhizoids of the green algae Chara, positively gravitropic growth is governed by statoliths kept in a dynamically stable position 10–25 μm above the cell tip by a complex interaction of gravity and actomyosin forces. Any deviation of the tube-like cells from the tip-downward orientation causes statoliths to sediment onto the gravisensitive subapical cell flank which initiates a gravitropic curvature response. Microgravity experiments have shown that abolishing the net tip-directed gravity force results in an actomyosin-mediated axial displacement of statoliths away from the cell tip. The present study was performed to critically assess the quality of microgravity simulation provided by different operational modes of a Random Positioning Machine (RPM) running with one axis (2D mode) or two axes (3D mode) and different rotational speeds (2D), speed ranges and directions (3D). The effects of 2D and 3D rotation were compared with data from experiments in real microgravity conditions (MAXUS sounding rocket missions). Rotational speeds in the range of 60–85 rpm in 2D and 3D modes resulted in a similar kinetics of statolith displacement as compared to real microgravity data, while slower clinorotation (2–11 rpm) caused a reduced axial displacement and a more dispersed arrangement of statoliths closer to the cell tip. Increasing the complexity of rotation by adding a second rotation axis in case of 3D clinorotation did not increase the quality of microgravity simulation, however, increased side effects such as the level of vibrations resulting in a more dispersed arrangement of statoliths. In conclusion, fast 2D clinorotation provides the most appropriate microgravity simulation for investigating the graviperception mechanism in Chara rhizoids, whereas slower clinorotation speeds and rotating samples around two axes do not improve the quality of microgravity simulation.

Item URL in elib:https://elib.dlr.de/119274/
Document Type:Article
Title:Analysis of Statoliths Displacement in Chara Rhizoids for Validating the Microgravity-Simulation Quality of Clinorotation Modes
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Krause, LarsGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germany and Forschungszentrum Juelich GmbH, Agrosphere (IBG-3) Institute of Bio- and Geosciences, Juelich, Germanyhttps://orcid.org/0000-0002-2521-4959
Braun, MarkusUniversity of Bonn, Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), Gravitational Biology, Bonn, Germany and German Aerospace Center (DLR), Space Administration, Bonn, GermanyUNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Date:12 January 2018
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:30
DOI :10.1007/s12217-017-9580-7
Page Range:pp. 229-236
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Chara, Clinorotation, Gravitropism, Microgravity, Random positioning machine (RPM), Sounding rockets
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung, R - Vorhaben Biowissenschaftliche Exp.-vorbereitung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Space Administration
Deposited By: Duwe, Helmut
Deposited On:23 May 2018 13:33
Last Modified:23 Feb 2019 00:22

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