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How do Bacillus subtilis spores resist to ultra-high vacuum?

Cortesao, M. and Moeller, R. (2017) How do Bacillus subtilis spores resist to ultra-high vacuum? In: 19th INTERNATIONAL CONFERENCE on Bacilli & Gram-Positive Bacteria - Abstractbook. 19th INTERNATIONAL CONFERENCE on Bacilli & Gram-Positive Bacteria, 11–15 June 2017, Berlin, Germany.

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Spore forming bacteria, such as Bacillus subtilis, are considered of great importance in space-life sciences, mainly because of the spores" multi-resistant characteristics. This sets spores as potential contaminators, posing a threat to food preservation as well as to planetary protection. It is the spores" overall structure that enables them to be so resilient, and efforts to study their resistance mechanisms to several space conditions have been undertaken. However, the effects of ultra-high vacuum in spores´ viability are not well-known. Thus, it is our interest to open the spores" molecular treasure box and unravel the molecular mechanisms of spores´ resistance to desiccation conditions in ultra-high vacuum. For that, air-dried spores of B. subtilis, representing a wide set of 28 strains, were immobilized on stainless steel discs and exposed to ultra-high vacuum for 7 days. Each strain counts with specific knockout genes associated with numerous known molecular mechanisms of spore resistance to different space conditions: SASP (sspA, sspB, sspE), DPA (spoVF), core water content (dacB), spore coat layer (cotE, safA, cotE safA), spore crust (cotX cotYZ, cotVW), DNA repair by HR (homologous recombination), NHEJ (non-homologous end-joining), BER (base excision repair) (recA, ku ligD, nfo, exoA) and detoxification (mrgA, katX). Spore resistance to ultra-high vacuum showed to be mainly dependent on SASPs, which are crucial in DNA protection. Moreover, DNA repair in the exposed spores showed to involve mostly ExoA Nfo, meaning BER plays a more important role than HR (RecA), NHEJ and detoxification (KatX).

Item URL in elib:https://elib.dlr.de/118086/
Document Type:Conference or Workshop Item (Poster)
Title:How do Bacillus subtilis spores resist to ultra-high vacuum?
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Cortesao, M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Moeller, R.UNSPECIFIEDhttps://orcid.org/0000-0002-2371-0676UNSPECIFIED
Journal or Publication Title:19th INTERNATIONAL CONFERENCE on Bacilli & Gram-Positive Bacteria - Abstractbook
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In ISI Web of Science:No
Keywords:Bacillus subtilis, ultra-high vacuum
Event Title:19th INTERNATIONAL CONFERENCE on Bacilli & Gram-Positive Bacteria
Event Location:Berlin, Germany
Event Type:international Conference
Event Dates:11–15 June 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jan 2018 13:43
Last Modified:31 Jul 2019 20:15

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