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Influence of simulated microgravity on B. subtilis biofilms

Fuchs, F. M. and Holland, G. and Madela, K. and Falcón García, C. and Hemmersbach, R. and Laue, M. and Lieleg, O. and Moeller, R. (2017) Influence of simulated microgravity on B. subtilis biofilms. In: BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe (D13808), p. 318. Springer. MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM, 05-08-March 2017, Würzburg, Germany. ISSN 0947-0867

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Abstract

Bacillus subtilis is one of the most studied Gram positive model organisms. Since mission Apollo 16, B. subtilis has been used for a multitude of space experiments. Investigating the influence of extreme conditions like those in space, non-domesticated strains, such as NCIB 3610 are of special interest regarding their ability to form biofilms. Since it is known that planktonic life is the exception, biofilms are considered as predominant way of living (Moons et al., 2009). Biofilms are organized in a complex selfproduced extracellular polymeric matrix commonly composed of polysaccharides, proteins and nucleic acids. Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is a problem, not only in industry and medicine, but it can be problematic under spaceflight conditions. Especially the loss of gravity coupled with changed levels of radiation might influence the resistance and therefore the virulence of bacterial biofilms. This can possibly evoke problems for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used the biofilm-forming wildtype strain NCIB 3610 and a biofilm-matrix deficient mutant (deletion of 15-gene exopolysaccharide operon, epsA-O) to study the impact of reduced gravity on maturated biofilms. Our major research goal is to compare biofilm formation in simulated microgravity (using a 2D clinostat) to terrestrial gravity (1g) conditions by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and inner structure, respectively. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography.

Item URL in elib:https://elib.dlr.de/118080/
Document Type:Conference or Workshop Item (Poster)
Title:Influence of simulated microgravity on B. subtilis biofilms
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fuchs, F. M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germanyhttps://orcid.org/0000-0001-5669-5655
Holland, G.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Madela, K.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Falcón García, C.Technische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Hemmersbach, R.Gravitational Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715
Laue, M.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Lieleg, O.Technische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:2017
Journal or Publication Title:BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Page Range:p. 318
Publisher:Springer
ISSN:0947-0867
Status:Published
Keywords:Microgravity, Microbiology, Bacillus subtilis
Event Title:MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM
Event Location:Würzburg, Germany
Event Type:international Conference
Event Dates:05-08-March 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie, R - Vorhaben Artificial Gravity
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jan 2018 13:42
Last Modified:31 Jul 2019 20:15

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