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Influence of simulated microgravity on B. subtilis biofilms

Fuchs, F. M. und Holland, G. und Madela, K. und Falcón García, C. und Hemmersbach, R. und Laue, M. und Lieleg, O. und Moeller, R. (2017) Influence of simulated microgravity on B. subtilis biofilms. In: BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe (D13808), Seite 318. Springer. MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM, 05-08-March 2017, Würzburg, Germany. ISSN 0947-0867.

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Kurzfassung

Bacillus subtilis is one of the most studied Gram positive model organisms. Since mission Apollo 16, B. subtilis has been used for a multitude of space experiments. Investigating the influence of extreme conditions like those in space, non-domesticated strains, such as NCIB 3610 are of special interest regarding their ability to form biofilms. Since it is known that planktonic life is the exception, biofilms are considered as predominant way of living (Moons et al., 2009). Biofilms are organized in a complex selfproduced extracellular polymeric matrix commonly composed of polysaccharides, proteins and nucleic acids. Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is a problem, not only in industry and medicine, but it can be problematic under spaceflight conditions. Especially the loss of gravity coupled with changed levels of radiation might influence the resistance and therefore the virulence of bacterial biofilms. This can possibly evoke problems for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used the biofilm-forming wildtype strain NCIB 3610 and a biofilm-matrix deficient mutant (deletion of 15-gene exopolysaccharide operon, epsA-O) to study the impact of reduced gravity on maturated biofilms. Our major research goal is to compare biofilm formation in simulated microgravity (using a 2D clinostat) to terrestrial gravity (1g) conditions by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and inner structure, respectively. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography.

elib-URL des Eintrags:https://elib.dlr.de/118080/
Dokumentart:Konferenzbeitrag (Poster)
Titel:Influence of simulated microgravity on B. subtilis biofilms
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Fuchs, F. M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germanyhttps://orcid.org/0000-0001-5669-5655NICHT SPEZIFIZIERT
Holland, G.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Madela, K.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Falcón García, C.Technische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Hemmersbach, R.Gravitational Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715NICHT SPEZIFIZIERT
Laue, M.Robert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Lieleg, O.Technische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676NICHT SPEZIFIZIERT
Datum:2017
Erschienen in:BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Seitenbereich:Seite 318
Verlag:Springer
ISSN:0947-0867
Status:veröffentlicht
Stichwörter:Microgravity, Microbiology, Bacillus subtilis
Veranstaltungstitel:MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM
Veranstaltungsort:Würzburg, Germany
Veranstaltungsart:internationale Konferenz
Veranstaltungsdatum:05-08-March 2017
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie (alt), R - Vorhaben Artificial Gravity (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:11 Jan 2018 13:42
Letzte Änderung:31 Jul 2019 20:15

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