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Understanding the molecular mechanisms involved in the spore inactivation by plasma sterilization

Raguse, M. and Fiebrandt, M. and Stapelmann, K. and Awakowicz, P. and Eaton, P. and Eichenberger, P. and Douki, T. and Moeller, R. (2017) Understanding the molecular mechanisms involved in the spore inactivation by plasma sterilization. In: BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe (D13808), p. 221. Springer. MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM, 05-08-March 2017, Würzburg, Germany. ISSN 0947-0867

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Abstract

Being the most resistant form of a biological system, spores of Bacillus subtilis are very resistant against a broad spectrum of sterilization methods and, therefore, are commonly used as a biological indicator in order to verify the functionality of a sterilization procedure. The process of low-pressure plasma sterilization is a promising alternative to conventional sterilization methods as it is extremely fast, efficient and gentle to heatsensitive materials. Active plasma species contain a high degree of sporicidal UV/VUV-radiation, as well as charged particles and free radicals, which exert detrimental effects on microorganisms. In this study we present novel insights into the key factors involved in spore inactivation by low pressure plasma sterilization using a double inductively-coupled plasma reactor. In order to standardize the assessment of spore inactivation efficiencies by plasma discharges, an electrically operated deposition device was developed, allowing fast, reproducible, and homogeneous preparation of B. subtilis spore in monolayers on surfaces leading to more reliable investigations. We demonstrate that low-pressure plasma discharges of argon and oxygen discharges cause significant physical damage to spore surface structures as visualized by atomic force microscopy. A systematic analysis of B. subtilis spores lacking individual coat and crust layers - the first barrier to environmental influences – revealed the coat to be a major factor in spore resistance towards plasma treatment (Raguse et al., 2016). In order to gain a better understanding of the complex molecular mechanisms involved in the inactivation by plasma sterilization processes, we analyzed plasma-induced DNA lesions in vitro, identified general and spore-specific DNA lesions, and characterized different DNA repair mechanisms during spore revival after plasma treatment.

Item URL in elib:https://elib.dlr.de/118078/
Document Type:Conference or Workshop Item (Poster)
Title:Understanding the molecular mechanisms involved in the spore inactivation by plasma sterilization
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Raguse, M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, GermanyUNSPECIFIED
Fiebrandt, M.Ruhr-Universität Bochum , Lehrstuhl für Allgemeine Elektrotechnik und Plasmatechnik, Bochum, GermanyUNSPECIFIED
Stapelmann, K.Ruhr-Universität Bochum , Lehrstuhl für Allgemeine Elektrotechnik und Plasmatechnik, Bochum, GermanyUNSPECIFIED
Awakowicz, P.Ruhr-Universität Bochum , Lehrstuhl für Allgemeine Elektrotechnik und Plasmatechnik, Bochum, GermanyUNSPECIFIED
Eaton, P.University of Porto, Faculty of Sciences, Porto, PortugalUNSPECIFIED
Eichenberger, P.New York University, Center of Genomics and System Biology, New York, New YorkUNSPECIFIED
Douki, T.CEA-Grenoble, Service de Chimie Inorganique et Biologique, Grenoble, FranceUNSPECIFIED
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (dlr), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:2017
Journal or Publication Title:BIOspektrum - Das Magazin für Biowissenschaften - Abstractbook - Sonderausgabe
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Page Range:p. 221
Publisher:Springer
ISSN:0947-0867
Status:Published
Keywords:Microbiology, spore resistance, planetary protection, Plasma sterilization
Event Title:MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM
Event Location:Würzburg, Germany
Event Type:international Conference
Event Dates:05-08-March 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jan 2018 13:42
Last Modified:31 Jul 2019 20:15

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