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Influence of simulated microgravity on B. subtilis biofilms

Fuchs, Felix M. and Holland, Gudrun and Madela, Kazimierz and Falcón García, Carolina and Hemmersbach, Ruth and Laue, Michael and Lieleg, Oliver and Moeller, Ralf (2017) Influence of simulated microgravity on B. subtilis biofilms. In: BIOSPEKTRUM - ABSTRACTBOOK MICROBIOLOGY AND INFECTION 2017 (D13808). Springer. MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM, March 05-08, 2017, Würzburg, Germany. ISSN 0947-0867

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Abstract

Bacillus subtilis is one of the most studied Gram positive model organisms. Since mission Apollo 16, B. subtilis has been used for a multitude of space experiments. Investigating the influence of extreme conditions like those in space, non-domesticated strains, such as NCIB 3610 are of special interest regarding their ability to form biofilms. Since it is known that planktonic life is the exception, biofilms are considered as predominant way of living (Moons et al., 2009). Biofilms are organized in a complex self-produced extracellular polymeric matrix commonly composed of polysaccharides, proteins and nucleic acids. Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is a problem, not only in industry and medicine, but it can be problematic under spaceflight conditions. Especially the loss of gravity coupled with changed levels of radiation might influence the resistance and therefore the virulence of bacterial biofilms. This can possibly evoke problems for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used the biofilm-forming wildtype strain NCIB 3610 and a biofilm-matrix deficient mutant (deletion of 15-gene exopolysaccharide operon, epsA-O) to study the impact of reduced gravity on maturated biofilms. Our major research goal is to compare biofilm formation in simulated microgravity (using a 2D clinostat) to terrestrial gravity (1g) conditions by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and inner structure, respectively. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography.

Item URL in elib:https://elib.dlr.de/117719/
Document Type:Conference or Workshop Item (Speech)
Title:Influence of simulated microgravity on B. subtilis biofilms
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fuchs, Felix M.German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), Germany; Felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655
Holland, GudrunRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Madela, KazimierzRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Falcón García, CarolinaTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Hemmersbach, Ruth3German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-5308-6715
Laue, MichaelRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Lieleg, OliverTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:2017
Journal or Publication Title:BIOSPEKTRUM - ABSTRACTBOOK MICROBIOLOGY AND INFECTION 2017
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Publisher:Springer
ISSN:0947-0867
Status:Published
Keywords:biofilm, B. subtilis, whole biofilm analysis, microgravity, SEM, TEM, CLSM, profilometry
Event Title:MICROBIOLOGY AND INFECTION 2017, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM
Event Location:Würzburg, Germany
Event Type:international Conference
Event Dates:March 05-08, 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Artificial Gravity, R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jan 2018 13:40
Last Modified:31 Jul 2019 20:15

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