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Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity

Fuchs, Felix M. and Holland, Gudrun and Madela, Kazimierz and Falcón García, Carolina and Hemmersbach, Ruth and Laue, Michael and Lieleg, Oliver and Moeller, Ralf (2017) Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity. 19th International Conference on Bacilli & Gram-Positive Bacteria, 11.-15. Juni, 2017, Berlin, Germany.

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Abstract

Since humans are travelling into the outer atmospheres of our planet and beyond, a new environmental habitat was established. Knowing that life - possibly since its first appearance on Earth is adapted to terrestrial gravity, the impact of microgravity on prokaryotes and humans remains mostly unclear. Investigating the influence of extreme conditions like those in space, Bacillus subtilis was chosen as model organism. Non-domesticated strains, such as NCIB 3610 have the ability to form biofilms as well as highly resistant endospores. Since it is known that planktonic life is the exception, biofilms are considered as predominant way of living (Moons et al., 2009). Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is a problem, not only in industry and medicine, but it can be problematic during spaceflight conditions, especially for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used a rotating 2D-clinostat to determine the effect of simulated microgravity (sim.-μg) and terrestrial gravity (1g) on B. subtilis biofilms and spores by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and inner structure, respectively. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography. In order to test the resistance of spores grown under simulated gravity, germination assays, as well as survival assays were used. First results revealed that spores grown under the influence of sim.-μg had an increased spontaneous germination rate compared to spores grown at 1g.

Item URL in elib:https://elib.dlr.de/117704/
Document Type:Conference or Workshop Item (Poster)
Title:Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fuchs, Felix M.German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology, Space Microbiology Research Group, Cologne (Köln), Germany; Felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655
Holland, GudrunRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Madela, KazimierzRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Falcón García, CarolinaTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Laue, MichaelRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Lieleg, OliverTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyUNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology, Space Microbiology Research Group, Cologne (Köln), Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Date:2017
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Bacillus subtilis, microgravity
Event Title:19th International Conference on Bacilli & Gram-Positive Bacteria
Event Location:Berlin, Germany
Event Type:international Conference
Event Dates:11.-15. Juni, 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Artificial Gravity, R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:11 Jan 2018 13:39
Last Modified:31 Jul 2019 20:15

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