elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Backward translation to the cellular Level: Studies under artificial gravity

Hemmersbach, Ruth and Liemersdorf, Christian and Brungs, Sonja and Waßer, Kai and Hauslage, Jens and Jordan, Jens (2017) Backward translation to the cellular Level: Studies under artificial gravity. 21. IAA Humans in Space Symposium 2017, 27. – 30. Nov. 2017, Shenzhen, China.

Full text not available from this repository.

Official URL: http://www.his2017.com.cn/

Abstract

Suitable platforms providing well defined and reproducible artificial gravity conditions, particularly simulated microgravity and hypergravity, have a pivotal role in preparing space-based experiments. Clinostats and Random Positioning Machines (RPM) are commonly applied in gravitational cell biology to simulate microgravity conditions. To exclude potential non-gravitational cellular responses to both methodologies, we applied the highly sensitive reporter organism, the dinoflagellate Pyrocystis noctiluca. The organism responds to shear stress with detectable bioluminescence emission. Pyrocystis noctiluca was exposed to different microgravity simulations while varying operational modes. With cells in a RPM rotating around two axes with random velocities and directions, we observed significantly greater mechanical stress compared with clinostat experiments applying constant rotation around one axis. We conclude that in contrast to RPM, one axis clinorotation induces substantially less non-gravitational responses through shear forces. Therefore, we apply clinostats as our preferred and validated method for the simulation of microgravity in ongoing cellular experiments. Altered gravity conditions elicit physiological changes in the human body. Translation from humans to cells will help identifying fundamental gravity-related mechanisms and targets for preventative measures. Our cell models include stem cells, neurons, glia, cardiac muscle, and immune cells. Case studies applying a broad range of ground-based artificial gravity facilities, which are available in our research platform :envihab, will be presented.

Item URL in elib:https://elib.dlr.de/116912/
Document Type:Conference or Workshop Item (Speech)
Title:Backward translation to the cellular Level: Studies under artificial gravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Liemersdorf, Christiangravitational biology, institute of aerospace medicine, german aerospace centre (dlr), cologne, germanyhttps://orcid.org/0000-0001-8407-5226
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Waßer, Kaigerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Jordan, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Date:27 November 2017
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Cellular level, Artificial gravity, RPM, Clinorotation, Pyrocystis noctiluca
Event Title:21. IAA Humans in Space Symposium 2017
Event Location:Shenzhen, China
Event Type:international Conference
Event Dates:27. – 30. Nov. 2017
Organizer:International Astronautical Academy (IAA)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Nutzerunterstützung Biowiss. Bodenprogramm
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine
Deposited By: Duwe, Helmut
Deposited On:14 Dec 2017 09:15
Last Modified:14 Dec 2017 09:15

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.