Hemmersbach, Ruth and Liemersdorf, Christian and Brungs, Sonja and Waßer, Kai and Hauslage, Jens and Jordan, Jens (2017) Backward translation to the cellular Level: Studies under artificial gravity. 21. IAA Humans in Space Symposium 2017, 2017-11-27 - 2017-11-30, Shenzhen, China.
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Official URL: http://www.his2017.com.cn/
Abstract
Suitable platforms providing well defined and reproducible artificial gravity conditions, particularly simulated microgravity and hypergravity, have a pivotal role in preparing space-based experiments. Clinostats and Random Positioning Machines (RPM) are commonly applied in gravitational cell biology to simulate microgravity conditions. To exclude potential non-gravitational cellular responses to both methodologies, we applied the highly sensitive reporter organism, the dinoflagellate Pyrocystis noctiluca. The organism responds to shear stress with detectable bioluminescence emission. Pyrocystis noctiluca was exposed to different microgravity simulations while varying operational modes. With cells in a RPM rotating around two axes with random velocities and directions, we observed significantly greater mechanical stress compared with clinostat experiments applying constant rotation around one axis. We conclude that in contrast to RPM, one axis clinorotation induces substantially less non-gravitational responses through shear forces. Therefore, we apply clinostats as our preferred and validated method for the simulation of microgravity in ongoing cellular experiments. Altered gravity conditions elicit physiological changes in the human body. Translation from humans to cells will help identifying fundamental gravity-related mechanisms and targets for preventative measures. Our cell models include stem cells, neurons, glia, cardiac muscle, and immune cells. Case studies applying a broad range of ground-based artificial gravity facilities, which are available in our research platform :envihab, will be presented.
Item URL in elib: | https://elib.dlr.de/116912/ | ||||||||||||||||||||||||||||
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Document Type: | Conference or Workshop Item (Speech) | ||||||||||||||||||||||||||||
Title: | Backward translation to the cellular Level: Studies under artificial gravity | ||||||||||||||||||||||||||||
Authors: |
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Date: | 27 November 2017 | ||||||||||||||||||||||||||||
Refereed publication: | No | ||||||||||||||||||||||||||||
Open Access: | No | ||||||||||||||||||||||||||||
Gold Open Access: | No | ||||||||||||||||||||||||||||
In SCOPUS: | No | ||||||||||||||||||||||||||||
In ISI Web of Science: | No | ||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||
Keywords: | Cellular level, Artificial gravity, RPM, Clinorotation, Pyrocystis noctiluca | ||||||||||||||||||||||||||||
Event Title: | 21. IAA Humans in Space Symposium 2017 | ||||||||||||||||||||||||||||
Event Location: | Shenzhen, China | ||||||||||||||||||||||||||||
Event Type: | international Conference | ||||||||||||||||||||||||||||
Event Start Date: | 27 November 2017 | ||||||||||||||||||||||||||||
Event End Date: | 30 November 2017 | ||||||||||||||||||||||||||||
Organizer: | International Astronautical Academy (IAA) | ||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Nutzerunterstützung Biowiss. Bodenprogramm (old) | ||||||||||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Gravitational Biology Institute of Aerospace Medicine | ||||||||||||||||||||||||||||
Deposited By: | Duwe, Helmut | ||||||||||||||||||||||||||||
Deposited On: | 14 Dec 2017 09:15 | ||||||||||||||||||||||||||||
Last Modified: | 24 Apr 2024 20:21 |
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