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Bioassays for validation of ground-based microgravity simulators

Hemmersbach, Ruth and Brungs, Sonja and Waßer, Kai and Hauslage, Jens (2017) Bioassays for validation of ground-based microgravity simulators. 25th European Low Gravity Research Association Biennial Symposium 2017, 2. – 6. Oktober 2017, Antibes, Juan-les-Pins, Frankreich.

Full text not available from this repository.

Official URL: http://www.elgra.org/?page_id=464

Abstract

Well defined and reproducible conditions are a prerequisite for platforms such as clinostats and Random Positioning Machines (RPM) in order to provide simulated microgravity and to prepare space-based experiments. To visualize potential non-gravitational cellular responses during microgravity simulation, we applied the dinoflagellate Pyrocystis noctiluca as a highly sensitive reporter organism. This organism responds to shear stress with detectable bioluminescence emission. Pyrocystis noctiluca was exposed to different microgravity simulations while varying operational modes. With cells in a RPM rotating around two axes with random velocities and directions, we observed significantly greater mechanical stress compared with clinostat experiments applying constant rotation around one axis. Thus, we conclude that in contrast to RPM, one axis clinorotation induces substantially less non-gravitational responses through shear forces. Therefore, we apply clinostats as our preferred and validated method for the simulation of microgravity in ongoing cellular experiments. Examples will be presented.

Item URL in elib:https://elib.dlr.de/116908/
Document Type:Conference or Workshop Item (Speech)
Title:Bioassays for validation of ground-based microgravity simulators
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Waßer, Kaigerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Date:2 October 2017
Refereed publication:No
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Bioassays, Microgravity Simulation, RPM, Clinorotation
Event Title:25th European Low Gravity Research Association Biennial Symposium 2017
Event Location:Antibes, Juan-les-Pins, Frankreich
Event Type:international Conference
Event Dates:2. – 6. Oktober 2017
Organizer:European Low Gravity Research Association (ELGRA)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Nutzerunterstützung Biowiss. Bodenprogramm, R - Projekt :envihab, R - Project eu:cropis
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:14 Dec 2017 09:12
Last Modified:14 Dec 2017 09:12

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