Hemmersbach, Ruth and Brungs, Sonja and Waßer, Kai and Hauslage, Jens (2017) Bioassays for validation of ground-based microgravity simulators. 25th European Low Gravity Research Association Biennial Symposium 2017, 2017-10-02 - 2017-10-06, Antibes, Juan-les-Pins, Frankreich.
Full text not available from this repository.
Official URL: http://www.elgra.org/?page_id=464
Abstract
Well defined and reproducible conditions are a prerequisite for platforms such as clinostats and Random Positioning Machines (RPM) in order to provide simulated microgravity and to prepare space-based experiments. To visualize potential non-gravitational cellular responses during microgravity simulation, we applied the dinoflagellate Pyrocystis noctiluca as a highly sensitive reporter organism. This organism responds to shear stress with detectable bioluminescence emission. Pyrocystis noctiluca was exposed to different microgravity simulations while varying operational modes. With cells in a RPM rotating around two axes with random velocities and directions, we observed significantly greater mechanical stress compared with clinostat experiments applying constant rotation around one axis. Thus, we conclude that in contrast to RPM, one axis clinorotation induces substantially less non-gravitational responses through shear forces. Therefore, we apply clinostats as our preferred and validated method for the simulation of microgravity in ongoing cellular experiments. Examples will be presented.
Item URL in elib: | https://elib.dlr.de/116908/ | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Document Type: | Conference or Workshop Item (Speech) | ||||||||||||||||||||
Title: | Bioassays for validation of ground-based microgravity simulators | ||||||||||||||||||||
Authors: |
| ||||||||||||||||||||
Date: | 2 October 2017 | ||||||||||||||||||||
Refereed publication: | No | ||||||||||||||||||||
Open Access: | No | ||||||||||||||||||||
Gold Open Access: | No | ||||||||||||||||||||
In SCOPUS: | No | ||||||||||||||||||||
In ISI Web of Science: | No | ||||||||||||||||||||
Status: | Published | ||||||||||||||||||||
Keywords: | Bioassays, Microgravity Simulation, RPM, Clinorotation | ||||||||||||||||||||
Event Title: | 25th European Low Gravity Research Association Biennial Symposium 2017 | ||||||||||||||||||||
Event Location: | Antibes, Juan-les-Pins, Frankreich | ||||||||||||||||||||
Event Type: | international Conference | ||||||||||||||||||||
Event Start Date: | 2 October 2017 | ||||||||||||||||||||
Event End Date: | 6 October 2017 | ||||||||||||||||||||
Organizer: | European Low Gravity Research Association (ELGRA) | ||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Nutzerunterstützung Biowiss. Bodenprogramm (old), R - Projekt :envihab (old), R - Project eu:cropis (old) | ||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Gravitational Biology | ||||||||||||||||||||
Deposited By: | Duwe, Helmut | ||||||||||||||||||||
Deposited On: | 14 Dec 2017 09:12 | ||||||||||||||||||||
Last Modified: | 24 Apr 2024 20:21 |
Repository Staff Only: item control page