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Biochip-based instruments development for space exploration: influence of the antibody immobilization process on the biochip resistance to freeze-drying, temperature shifts and cosmic radiations

Coussot, G. and Moreau, T and Faye, Clément and Vigier, F and Baque, Mickael and Le Postollec, A. and Incerti, S. and Dobrijevic, M. and Vandenabeele-Trambouze, O. (2017) Biochip-based instruments development for space exploration: influence of the antibody immobilization process on the biochip resistance to freeze-drying, temperature shifts and cosmic radiations. International Journal of Astrobiology, 16 (2), pp. 190-199. Cambridge University Press. DOI: 10.1017/S1473550416000173 ISSN 1473-5504

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Official URL: https://www.cambridge.org/core/journals/international-journal-of-astrobiology/article/biochipbased-instruments-development-for-space-exploration-influence-of-the-antibody-immobilization-process-on-the-biochip-resistance-to-freezedrying-temperature-shifts-

Abstract

Due to the diversity of antibody (Ab)-based biochips chemistries available and the little knowledge about biochips resistance to space constraints, immobilization of Abs on the surface of the biochips dedicated to Solar System exploration is challenging. In the present paper, we have developed ten different biochip models including covalent or affinity immobilization with full-length Abs or Ab fragments. Ab immobilizations were carried out in oriented/non-oriented manner using commercial activated surfaces with N-hydroxysuccinic ester (NHS-surfaces) or homemade surfaces using three generations of dendrimers (dendrigraft of poly L-lysine (DGL) surfaces). The performances of the Ab -based surfaces were cross-compared on the following criteria: (i) analytical performances (expressed by both the surface density of immobilized Abs and the amount of antigens initially captured by the surface) and (ii) resistance of surfaces to preparation procedure (freeze-drying, storage) or spatial constraints (irradiation and temperature shifts) encountered during a space mission. The latter results have been expressed as percentage of surface binding capacity losses (or percentage of remaining active Abs). The highest amount of captured antigen was achieved with Ab surfaces having full-length Abs and DGL-surfaces that have much higher surface densities than commercial NHS-surface. After freeze-drying process, thermal shift and storage sample exposition, we found that more than 80% of surface binding sites remained active in this case. In addition, the resistance of Ab surfaces to irradiation with particles such as electron, carbon ions or protons depends not only on the chemistries (covalent/affinity linkages) and strategies (oriented/non-oriented) used to construct the biochip, but also on the type, energy and fluence of incident particles. Our results clearly indicate that full-length Ab immobilization on NHS-surfaces and DGL-surfaces should be preferred for potential use in instruments for planetary exploration.

Item URL in elib:https://elib.dlr.de/116386/
Document Type:Article
Title:Biochip-based instruments development for space exploration: influence of the antibody immobilization process on the biochip resistance to freeze-drying, temperature shifts and cosmic radiations
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Coussot, G.institut des biomolécules max mousseron-ibmm, centre national de la recherche scientifique, université de montpellier 1, université de montpellier 2, unité mixte de recherche 5247, place e. bataillon, cc17006, 34095 montpellier cedex 5, franceUNSPECIFIED
Moreau, TCOLCOM, Cap Alpha, 34830 Clapiers, FranceUNSPECIFIED
Faye, ClémentCOLCOMUNSPECIFIED
Vigier, FGerman Aerospace Center (DLR), Institute of Planetary Research, Berlin, GermanyUNSPECIFIED
Baque, MickaelMickael.Baque (at) dlr.dehttps://orcid.org/0000-0002-6696-6030
Le Postollec, A.univ. bordeaux, lab, umr 5804, f-33270 floirac, france and cnrs, lab, umr 5804, f-33270 floirac, franceUNSPECIFIED
Incerti, S.université bordeaux 1, cnrs, in2p3, centre d’etudes nucléaires de bordeaux gradignan (cenbg), umr 5797, gradignan, franceUNSPECIFIED
Dobrijevic, M.univ. bordeaux, lab, umr 5804, f-33270 floirac, france and cnrs, lab, umr 5804, f-33270 floirac, franceUNSPECIFIED
Vandenabeele-Trambouze, O.institut des biomolécules max mousseron-ibmm, centre national de la recherche scientifique, université de montpellier 1, université de montpellier 2, unité mixte de recherche 5247, place e. bataillon, cc17006, 34095 montpellier cedex 5, franceUNSPECIFIED
Date:April 2017
Journal or Publication Title:International Journal of Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:16
DOI :10.1017/S1473550416000173
Page Range:pp. 190-199
Publisher:Cambridge University Press
ISSN:1473-5504
Status:Published
Keywords:Astrobiology, biochip, search for Extraterrestrial Life, space constraints
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Planetary Evolution and Life
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Deposited By: Baque, Dr. Mickael
Deposited On:30 Nov 2017 14:27
Last Modified:06 Sep 2019 15:15

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