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The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses

Beblo-Vranesevic, Kristina and Bohmeier, Maria and Perras, Alexandra K. and Schwendner, Petra and Rabbow, Elke and Moissl-Eichinger, Christine and Cockell, Charles S. and Pukall, Rüdiger and Vannier, Pauline and Marteinsson, Viggo T. and Monaghan, Euan P. and Ehrenfreund, Pascale and Garcia-Descalzo, Laura and Gómez, Felipe and Malki, Moustafa and Amils, Ricardo and Gaboyer, Frédéric and Westall, Frances and Cabezas, Patricia and Walter, Nicolas and Rettberg, Petra (2017) The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. PLoS One, 12 (10), e0185178. Public Library of Science (PLoS). DOI: 10.1371/journal.pone.0185178 ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0185178

Abstract

The limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we investigated the responses of a new isolate, Yersinia intermedia MASE-LG-1 to individual and combined stresses associated with the Martian surface. This organism belongs to an adaptable and persistent genus of anaerobic microorganisms found in many environments worldwide. The effects of desiccation, low pressure, ionizing radiation, varying temperature, osmotic pressure, and oxidizing chemical compounds were investigated. The strain showed a high tolerance to desiccation, with a decline of survivability by four orders of magnitude during a storage time of 85 days. Exposure to X-rays resulted in dose-dependent inactivation for exposure up to 600 Gy while applied doses above 750 Gy led to complete inactivation. The effects of the combination of desiccation and irradiation were additive and the survivability was influenced by the order in which they were imposed. Ionizing irradiation and subsequent desiccation was more deleterious than vice versa. By contrast, the presence of perchlorates was not found to significantly affect the survival of the Yersinia strain after ionizing radiation. These data show that the organism has the capacity to survive and grow in physical and chemical stresses, imposed individually or in combination that are associated with Martian environment. Eventually it lost its viability showing that many of the most adaptable anaerobic organisms on Earth would be killed on Mars today.

Item URL in elib:https://elib.dlr.de/115000/
Document Type:Article
Title:The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Beblo-Vranesevic, Kristinaradiation biology department, institute of aerospace medicine, german aerospace center (dlr), linder hoehe, 51147 cologne, germany; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121
Bohmeier, Mariaradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyUNSPECIFIED
Perras, Alexandra K.Department of Internal Medicine, Medical University of Graz, Graz, Austria and Department of Microbiology and Archaea, University of Regensburg, Regensburg, GermanyUNSPECIFIED
Schwendner, PetraSchool of Physics and Astronomy, UK Center for Astrobiology, University of Edinburgh, Edinburgh, United KingdomUNSPECIFIED
Rabbow, Elkeradiation biology department, institute of aerospace medicine, german aerospace center (dlr), linder hoehe, 51147 cologne, germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Moissl-Eichinger, ChristineDepartment of Internal Medicine, Medical University of Graz, Graz, Austria and BioTechMed Graz, Graz, AustriaUNSPECIFIED
Cockell, Charles S.School of Physics and Astronomy, UK Center for Astrobiology, University of Edinburgh, Edinburgh, United KingdomUNSPECIFIED
Pukall, RüdigerGerman Collection of Microorganisms and Cell Cultures, Leibniz Institute DSMZ, Braunschweig, GermanyUNSPECIFIED
Vannier, PaulineMATIS-Prokaria, Reykjavík, IcelandUNSPECIFIED
Marteinsson, Viggo T.MATIS-Prokaria, Reykjavík, Iceland and Faculty of Food Science and Nutrition, University of Iceland, Reykjavík, IcelandUNSPECIFIED
Monaghan, Euan P.Leiden Observatory, Universiteit Leiden, Leiden, NetherlandUNSPECIFIED
Ehrenfreund, PascaleLeiden Observatory, Universiteit Leiden, Leiden, Netherland and Space Policy Institute, George Washington University, Washington DC, United States of AmericaUNSPECIFIED
Garcia-Descalzo, LauraInstituto Nacional de Técnica Aeroespacial-Centro de Astrobiología (INTA-CAB), Madrid, SpainUNSPECIFIED
Gómez, FelipeInstituto Nacional de Técnica Aeroespacial-Centro de Astrobiología (INTA-CAB), Madrid, SpainUNSPECIFIED
Malki, MoustafaUniversidad Autónoma de Madrid (UAM), Madrid, SpainUNSPECIFIED
Amils, RicardoUniversidad Autónoma de Madrid (UAM), Madrid, SpainUNSPECIFIED
Gaboyer, FrédéricCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Orléans, FranceUNSPECIFIED
Westall, FrancesCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Orléans, FranceUNSPECIFIED
Cabezas, PatriciaEuropean Science Foundation (ESF), Strasbourg, FranceUNSPECIFIED
Walter, NicolasEuropean Science Foundation (ESF), Strasbourg, FranceUNSPECIFIED
Rettberg, Petraradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Date:2017
Journal or Publication Title:PLoS One
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:12
DOI :10.1371/journal.pone.0185178
Page Range:e0185178
Editors:
EditorsEmail
Amendola, RobertoENEA Centro Ricerche Casaccia, ITALY
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Status:Published
Keywords:anaerobic microorganisms, Mars, Yersinia intermedia MASE-LG-1
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:15 Nov 2017 15:41
Last Modified:08 Mar 2018 18:49

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