elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Bacillus subtilis DisA helps to circumvent replicative stress during spore revival

Raguse, Marina and Torres, Rubén and Seco, Elena M. and Gándara, Carolina and Ayora, Silvia and Moeller, Ralf and Alonso, Juan C. (2017) Bacillus subtilis DisA helps to circumvent replicative stress during spore revival. DNA Repair, 59, pp. 57-68. Elsevier. DOI: 10.1016/j.dnarep.2017.09.006 ISSN 1568-7864

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.dnarep.2017.09.006

Abstract

The mechanisms that allow to circumvent replicative stress, and to resume DNA synthesis are poorly understand in Bacillus subtilis. To study the role of the diadenylate cyclase DisA and branch migration translocase (BMT) RadA/Sms in restarting a stalled replication fork, we nicked and broke the circular chromosome of an inert mature haploid spore, damaged the bases, and measured survival of reviving spores. During undisturbed ripening, nicks and breaks should repaired by pathways that do not invoke long-range end resection or genetic exchange by homologous recombination, after which DNA replication might be initiated. We found that DNA damage reduced the viability of spores that lacked DisA, BMT (RadA/Sms, RuvAB or RecG), the Holliday junction resolvase RecU, or the translesion synthesis DNA polymerases (PolY1 or PolY2). DisA and RadA/Sms, in concert with RuvAB, RecG, RecU, PolY1 or PolY2, are needed to bypass replication-blocking lesions. DisA, which binds to stalled or reversed forks, did not apparently affect initiation of PriAdependent DNA replication in vitro. We propose that DisA is necessary to coordinate responses to replicative stress; they could help to circumvent damaged template bases that otherwise impede fork progression.

Item URL in elib:https://elib.dlr.de/114705/
Document Type:Article
Title:Bacillus subtilis DisA helps to circumvent replicative stress during spore revival
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Raguse, Marinaradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyUNSPECIFIED
Torres, RubénDepartamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNBCSIC), Darwin 3, Cantoblanco, 28049 Madrid, SpainUNSPECIFIED
Seco, Elena M.Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNBCSIC), Darwin 3, Cantoblanco, 28049 Madrid, SpainUNSPECIFIED
Gándara, CarolinaDepartamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNBCSIC), Darwin 3, Cantoblanco, 28049 Madrid, SpainUNSPECIFIED
Ayora, SilviaDepartamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNBCSIC), Darwin 3, Cantoblanco, 28049 Madrid, SpainUNSPECIFIED
Moeller, Ralfradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Alonso, Juan C.Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNBCSIC), Darwin 3, Cantoblanco, 28049 Madrid, Spain; jcalonso (at) cnb.csic.esUNSPECIFIED
Date:2017
Journal or Publication Title:DNA Repair
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:59
DOI :10.1016/j.dnarep.2017.09.006
Page Range:pp. 57-68
Publisher:Elsevier
ISSN:1568-7864
Status:Published
Keywords:Bacillus subtilis, diadenylate cyclase DisA
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:27 Oct 2017 12:58
Last Modified:27 Oct 2017 12:58

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.