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Metabolic response of Yersinia intermedia MASE-LG1 to osmotic stress

Schwendner, P. and Cockell, C. S. and Perras, A. and Rettberg, Petra and Beblo-Vranesevic, Kristina and Bohmeier, M. and Moissl-Eichinger, C. and Marteinsson, V. and Vannier, P. and Gomez, F. and Garcia-Descalzo, L. and Ehrenfreund, P. and Monaghan, E. P. and Westall, F. and Gaboyer, F. and Amils, R. and Malki, M. and Cabezas, P. and Walter, N. (2017) Metabolic response of Yersinia intermedia MASE-LG1 to osmotic stress. EANA 2017, 14.-17. August, 2017, Aarhus, Denmark.

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Abstract

Osmotic stress is one of the major limitations for cell growth. Microorganisms can evolve adaptations to abiotic stresses like high salt concentrations in the environment. Sensor and signal transduction networks provide information to the cell about the osmolarity of its surroundings leading to an immediate metabolic response to counteract the osmotic stress. Some of these adaptations can be structural, some are metabolic. Our current knowledge of microbial responses to osmotic challenges is based on studies of representative bacteria, archaea, and eukaryotic microbes that is mainly focusing on the effect of NaCl. There is still a lack of understanding whether and how different salts, for example NaCl versus MgSO4 alter the response of a microorganism to salt induced stress conditions. We chose Yersinia intermedia MASE-LG1 a strain isolated from an Icelandic lake as test organism. It is known for its abilities to adapt to a wide variety of habitats of rapidly changing environmental conditions. In order to identify which roles the different salts play in the global metabolic response, Y. intermedia was exposed sustained salt stress induced by either MgSO4 or NaCl. After metabolite extraction, metabolic profiles from three replicate cultures of Y. intermedia MASE-LG-1 grown under 3 different conditions (e.g. control salt stressed in MgSO4, and salt stressed in NaCl) were obtained. Generally, changes in numerous metabolites mainly in the amino acid metabolisms were observed in stressed samples compared to the control. To a lesser extent the carbohydrate metabolism was also affected. Looking at the effect of the different kations, the results clearly indicated significant differences in response to salt stress induced by the magnesium salt compared to sodium chloride. The results suggest that the amino acid synthesis, reflecting the general activity of translation operations, dominates the reaction to osmotic stress. These adaptations might provide necessary energy and building blocks to fuel processes conveying salt tolerance like the biosynthesis of compatible solutes. In addition we were able to identify metabolites which are linked to osmoprotective activity. The outcome of this study will have impact on our understanding of how microorganisms adapt to hostile environmental conditions.

Item URL in elib:https://elib.dlr.de/114438/
Document Type:Conference or Workshop Item (Speech)
Title:Metabolic response of Yersinia intermedia MASE-LG1 to osmotic stress
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Schwendner, P.UK Centre for Astrobiology, School of Physics and Astronomy, James Clerk Maxwell Building, The King’s Buildings, University of Edinburgh, Edinburgh, EH9 3FD, UK.UNSPECIFIED
Cockell, C. S.UK Centre for Astrobiology, School of Physics and Astronomy, James Clerk Maxwell Building, The King’s Buildings, University of Edinburgh, Edinburgh, EH9 3FD, UK.UNSPECIFIED
Perras, A.Medical University of Graz, Austria and University of Regensburg, Department for Microbiology, Universitätsstr. 31, 93053 Regensburg, Germany.UNSPECIFIED
Rettberg, Petraradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Beblo-Vranesevic, Kristinaradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyhttps://orcid.org/0000-0002-4834-7121
Bohmeier, M.radiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyUNSPECIFIED
Moissl-Eichinger, C.Medical University of Graz, Austria.UNSPECIFIED
Marteinsson, V.Matis ohf., Iceland.UNSPECIFIED
Vannier, P.Matis ohf., Iceland.UNSPECIFIED
Gomez, F.Centro de Astrobiologia (INTA-CSIC), Spain.UNSPECIFIED
Garcia-Descalzo, L.Centro de Astrobiologia (INTA-CSIC), Spain.UNSPECIFIED
Ehrenfreund, P.Leiden Observatory, Leiden University, The Netherlands.UNSPECIFIED
Monaghan, E. P.Leiden Observatory, Leiden University, The Netherlands.UNSPECIFIED
Westall, F.Centre National de La Recherche Scientifique (CNRS).UNSPECIFIED
Gaboyer, F.Centre National de La Recherche Scientifique (CNRS).UNSPECIFIED
Amils, R.Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain.UNSPECIFIED
Malki, M.Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain.UNSPECIFIED
Cabezas, P.European Science Foundation, France.UNSPECIFIED
Walter, N.European Science Foundation, France.UNSPECIFIED
Date:2017
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Yersinia intermedia MASE-LG1, osmotic stress
Event Title:EANA 2017
Event Location:Aarhus, Denmark
Event Type:international Conference
Event Dates:14.-17. August, 2017
Organizer:European Astrobiology Network Association
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:28 Sep 2017 11:48
Last Modified:31 Jul 2019 20:11

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