Garcia-Descalzo, L. and Gomez, F. and Cockell, C. S . and Schwendner, P. and Westall, F. and Gaboyer, F. and Rettberg, Petra and Beblo-Vranesevic, Kristina and Bohmeier, M. and Moissl-Eichinger, C. and Perras, A. and Amils, R. and Ehrenfreund, P. and Monaghan, E. and Marteinsson, V. and Vannier, P. and Malki, M. and Walter, N. and Cabezas, P. (2017) Preservation and detection of biomarkers in mineralized communities and its potential link to the variations in ORP. EANA 2017, 2017-08-14 - 2017-08-17, Aarhus, Denmark.
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Abstract
1. Introduction Physic-chemical processes of living organisms leave tell-tale signals in the environment. The search for these signatures is one of the main goals for Astrobiology and improving and optimizing its detection regarding Mars conditions is part of the MASE project objectives. Besides, the traces of some kinds of microorganisms can be well preserved, provided that they are rapidly mineralized and that the sediments in which they occur are rapidly cemented [1]. A developed antibody multiarray competitive immunoassay (MACIA) for the simultaneous detection of compounds of a wide range of molecular sizes or whole spores and cells [2] [3] is a suitable option for biomarker detection in samples with low biomass from Mars analogue sites as well as with biomineralized microorganism communi- ties. Moreover, biomineralization is often the first step of fossilization and produces particular chemical, structural and morphological features that can be preserved in fossil biominerals or microfossils [4] and some parameters as oxido-reduction potencial (ORP) or pH vary over the process. 2. Methods and objectives Samples from the three MASE campaigns in Iceland (Graenavatn Lake), United Kingdom (Boulby Mine) and Germany (Sippenauer Moor, Regensburg) and other one from an Alpin glacier were used to obtain enrichments and isolates as well as to extract and detect biomarkers in them. Some of the enrichments were exposed to mineralization to study, among others, the preservation of biosignatures by the assessment of antigen-antibody binding at different times. Simultaneously, the evolution of ORP through this process was monitored by two modules system (DTIVA: automated tools for microbial life detection) where ORP variations in those communities were followed through continuous measurements of nanosensors in closed chambers. An additional objective for MASE project is to develop a specific microarray with antibodies performanced from natural samples and isolates from MASE sampling sites. 3. Summary and Conclusions The presence of traces from some microbial metabolic groups were detected in the mineralized communities at three different times over the fossilization process. It was undertaken by us- ing a 168 antibody microarray for the immunoassay. There were observed variations in the resulting immunoprofiles. There seems to be a probably correlation between these changes and those in ORP through time. We consider that the simultaneous use of both approaches arises a promising tool to broaden our knowledge and improve the search for traces of life, present or past. Acknowledgements MASE is supported by European Community’s Seventh Framework Programme (FP7/2007-2013) under Grant Agreement n° 607297.
Item URL in elib: | https://elib.dlr.de/114345/ | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Document Type: | Conference or Workshop Item (Poster) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Title: | Preservation and detection of biomarkers in mineralized communities and its potential link to the variations in ORP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Authors: |
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Date: | 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Open Access: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Gold Open Access: | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In SCOPUS: | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Keywords: | MASE (Mars Analogues for Space Exploration) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Event Title: | EANA 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Event Location: | Aarhus, Denmark | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Event Type: | international Conference | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Event Start Date: | 14 August 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Event End Date: | 17 August 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Organizer: | European Astrobiology Network Association | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Strahlenbiologie (old) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Radiation Biology | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Deposited By: | Kopp, Kerstin | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Deposited On: | 25 Sep 2017 13:44 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Last Modified: | 24 Apr 2024 20:18 |
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