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NEXT-GENERATION SEQUENCING FOR MICROBIAL CHARACTERIZATION OF BIOVERMICULATIONS FROM A SULFURIC ACID CAVE IN APULIA (ITALY)

D’Angeli, Ilenia M. and De Waele, Jo and Ieva, Maria Grazia and Leuko, Stefan and Cappelletti, Martina and Parise, Mario and Saiz-Jimenez, Cesareo and Valme, Jurado (2017) NEXT-GENERATION SEQUENCING FOR MICROBIAL CHARACTERIZATION OF BIOVERMICULATIONS FROM A SULFURIC ACID CAVE IN APULIA (ITALY). 17th International Congress of Speleology, 23. - 29. Juli 2017, Sydney, Australia.

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Abstract

Sulfuric acid cave systems host abundant microbial communities that can colonize several environments displaying a variety of morphologies i.e. white filamentous mats floating on the water surface, white creamy moonmilk deposits on the walls, and biovermiculations. Up to date, only few reports have described the microbiological aspects behind biovermiculation geomicrobiology of Italian sulfuric acid caves despite their overall abundance in the territory. Here, we present the first characterization of biovermicalution microbial populations from Santa Cesarea Terme (Apulia, Italy) using a next-generation sequencing approach. In this area, four sulfuric acid caves located along the coastline of the Adriatic sea have been explored. The mixing of sea and upwelling acidic water characterizes these environments, where very peculiar microbiological communities are visible both floating on the water but also growing on cave walls. In particular, we focused our analysis on biovermiculations from Fetida cave. This cave opens at the sea level, and moving from the entrance to the deeper part, it is possible to observe a decrease of marine influence and a corresponding increase of the acidic effect of the upwelling waters. Biovermiculations copiously cover ceiling and walls mainly in the deeper and middle environments of the cave, while they are absent close to the entrance. From a morphological point of view, biovermiculations have a widespread range of colors going from grey to dark brown with an overall slimy appearance; dendritic morphologies alternate to very dense wall-covering sheets. Vermiculation samples from different locations within the cave (mid-section and the end) have been collected. Total DNA was extracted from each sample and 16S rDNA sequences were analysed through Ilumina MiSeq platform. The main lineages found in all the samples included Gammaproteobacteria, Betaproteobacteria, Alphaproteobacteria, Bacteroidetes, Planctomycetes Actinobacteria and Acidobacteria. In particular, the samples from the deeper part of the cave had the highest abundance of Acidobacteria and showed the presence of Epsilonproteobacteria that can be related to bacterial sulfur-oxidizing activity typically occurring in sulfidic environments.

Item URL in elib:https://elib.dlr.de/113956/
Document Type:Conference or Workshop Item (Speech)
Title:NEXT-GENERATION SEQUENCING FOR MICROBIAL CHARACTERIZATION OF BIOVERMICULATIONS FROM A SULFURIC ACID CAVE IN APULIA (ITALY)
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
D’Angeli, Ilenia M.Department of Biological, Geological and Environmental Sciences, University of Bologna, ItalyUNSPECIFIED
De Waele, JoDepartment of Biological, Geological and Environmental Sciences, University of Bologna, ItalyUNSPECIFIED
Ieva, Maria GraziaDepartment of Biological, Geological and Environmental Sciences, University of Bologna, ItalyUNSPECIFIED
Leuko, Stefanradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyhttps://orcid.org/0000-0002-0423-0896
Cappelletti, MartinaDepartment of Pharmacy and Biotechnology, University of Bologna, ItalyUNSPECIFIED
Parise, MarioCNR-IRPI Bari, via Amendola 122, ItalyUNSPECIFIED
Saiz-Jimenez, CesareoInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Sevilla, SpainUNSPECIFIED
Valme, JuradoInstituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Sevilla, SpainUNSPECIFIED
Date:2017
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:caves, microbial characterization
Event Title:17th International Congress of Speleology
Event Location:Sydney, Australia
Event Type:international Conference
Event Dates:23. - 29. Juli 2017
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:25 Sep 2017 13:54
Last Modified:31 Jul 2019 20:11

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