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The effects of heavy ion and X-ray exposure on SH-SY5Y cell differentiation

Mantas, Maria José Quintanilha (2017) The effects of heavy ion and X-ray exposure on SH-SY5Y cell differentiation. Master's, King’s College London.

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Background: Exposure to ionizing radiation (IR) has increased in the last century. One field where radiation is omnipresent and little is known regarding its effects on human health is spaceflight. With scheduled long-duration missions (LDM) to the Moon and Mars, there has been a push to investigate radiation hazards for various organs and tissues, including the central nervous system (CNS). IR may not only contribute to the increased incidence of neurodegenerative disorders, but also hamper neurogenesis, contributing to cognitive impairment and emotional instability during spaceflight. The aim of this study was to investigate the effects of ¹²C ion and X-ray exposure on neuroblastoma cell differentiation. Methods: SH-SY5Y cells were irradiated with ¹²C ions or X-rays. Neuronal differentiation was induced by incubation with retinoic acid (RA) and different concentrations of fetal bovine serum (FBS). The effects of radiation/differentiation on growth, cell-cycle distribution, morphology changes and presence of neuronal markers was assessed by cell count, flow cytometry, phase-contrast and fluorescence microscopy. Results: Stem-like and differentiated cells exposed to X-rays at 4Gy exhibited the lowest total/live cell numbers (p<0.01; p<0.001, respectively) and viability, and the highest fraction of dead cells, also comprising the largest sizes (p<0.01). RA inhibited growth (p<0.05), with no significant differences in other parameters. Cell cycle distribution was similar between conditions, however with increased G1-phase cells in RA-treated conditions and decreased S-phase cells in irradiated conditions (p<0.001). Morphological and immunohistochemical analysis indicated robust neurite outgrowth with intense BIII-tubulin in RA-differentiated cells. Irradiated cells lost their adherence phenotype and displayed balanced ratios of Nestin:BIII-tubulin, compared to the predominantly Nestin-positive control. Conclusion: ¹²C ions and X-rays irradiation did not induce differentiation of stem-like cells nor inhibit differentiation of surviving cells treated with RA.

Item URL in elib:https://elib.dlr.de/113948/
Document Type:Thesis (Master's)
Title:The effects of heavy ion and X-ray exposure on SH-SY5Y cell differentiation
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Mantas, Maria José Quintanilhainstitute of aerospace medicine, radiation biology division, german aerospace center (dlr e.v.), linder höhe, 51147 cologne, germanyUNSPECIFIED
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In ISI Web of Science:No
Number of Pages:82
Keywords:Ionizing Radiation, Neurons, Differentiation, Space missions
Institution:King’s College London
Department:Space Physiology & Health
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:25 Sep 2017 13:53
Last Modified:25 Sep 2017 13:53

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