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Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine

Warnke, Elisabeth and Pietsch, Jessica and Kopp, Sascha and Bauer, Johann and Sahana, Jayashree and Wehland, Markus and Krüger, Marcus and Hemmersbach, Ruth and Infanger, Manfred and Lützenberg, Roland and Grimm, Daniela (2017) Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine. Cellular Physiology and Biochemistry (43), pp. 257-270. S. Karger AG. DOI: 10.1159/00048036 ISSN 1015-8987

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Official URL: https://www.karger.com/Article/Pdf/480368

Abstract

Background/Aims: Spaceflight impacts on the function of the thyroid gland in vivo. In vitro normal and malignant thyrocytes assemble in part to multicellular spheroids (MCS) after exposure to the random positioning machine (RPM), while a number of cells remain adherent (AD). We aim to elucidate possible differences between AD and MCS cells compared to 1g-controls of normal human thyroid cells. Methods: Cells of the human follicular epithelial thyroid cell line Nthy-ori 3-1 were incubated for up to 72 h on the RPM. Afterwards, they were investigated by phase-contrast microscopy, quantitative real-time PCR and by determination of cytokines released in their supernatants. Results: A significant up-regulation of IL6, IL8 and CCL2 gene expression was found after a 4h RPM-exposure, when the whole population was still growing adherently. MCS and AD cells were detected after 24 h on the RPM. At this time, a significantly reduced gene expression in MCS compared to 1g-controls was visible for IL6, IL8, FN1, ITGB1, LAMA1, CCL2, and TLN1. After a 72 h RPM-exposure, IL-6, IL-8, and TIMP-1 secretion rates were increased significantly. Conclusion: Normal thyrocytes form MCS within 24 h. Cytokines seem to be involved in the initiation of MCS formation via focal adhesion proteins.

Item URL in elib:https://elib.dlr.de/113876/
Document Type:Article
Title:Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Warnke, Elisabethotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Pietsch, Jessicaotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Kopp, Saschauniversity of magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Bauer, Johannmax-planck institute for biochemistry, martinsried, germanyUNSPECIFIED
Sahana, JayashreeAarhus University, Institute of Biomedicine, Pharmacology, Aarhus, DenmarkUNSPECIFIED
Wehland, Markusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Krüger, Marcusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Infanger, Manfredotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Lützenberg, Rolandotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Grimm, Danielauniversity aarhus, department of biomedicine and pharmacology, aarhus, denmarkUNSPECIFIED
Date:30 August 2017
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1159/00048036
Page Range:pp. 257-270
Publisher:S. Karger AG
ISSN:1015-8987
Status:Published
Keywords:Thyroid cells, Multicellular spheroids, Cytokines, Focal adhesion, Extracellular matrix proteins, Pathway analyses
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:05 Sep 2017 15:57
Last Modified:23 Jan 2019 05:02

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