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Anaerobic microorganisms in astrobiological analogue environments: from field site to culture collection

Cockell, C. S. and Schwendner, P. and Perras, A. and Rettberg, Petra and Beblo-Vranesevic, Kristina and Bohmeier, M. and Rabbow, Elke and Moissl-Eichinger, C. and Wink, L. and Marteinsson, V. and Vannier, P. and Gomez, F. and Garcia-Descalzo, L. and Ehrenfreund, P. and Monaghan, E.P. and Westall, F. and Gaboyer, F. and Amils, R. and Malki, M. and Pukall, R. and Cabezas, P. and Walter, N. (2017) Anaerobic microorganisms in astrobiological analogue environments: from field site to culture collection. International Journal of Astrobiology, pp. 1-15. Cambridge University Press. DOI: 10.1017/S1473550417000246 ISSN 1473-5504

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1017/S1473550417000246

Abstract

Astrobiology seeks to understand the limits of life and to determine the physiology of organisms in order to better assess the habitability of other worlds. To successfully achieve these goals we require microorganisms from environments on Earth that approximate to extraterrestrial environments in terms of physical and/or chemical conditions. The most challenging of these environments with respect to sample collection, isolation and cultivation of microorganisms are anoxic environments. In this paper, an approach to this challenge was implemented within the European Union’s MASE (Mars Analogues for Space Exploration) project. In this review paper, we aim to provide a set of methods for future field work and sampling campaigns. A number of anoxic environment based on characteristics that make them analogous to past and present locations on Mars were selected. They included anoxic sulphur-rich springs (Germany), the salt-rich Boulby Mine (UK), a lake in a basaltic context (Iceland), acidic sediments in the Rio Tinto (Spain), glacier samples (Austria) and permafrost samples (Russia and Canada). Samples were collected under strict anoxic conditions to be used for cultivation and genomic community analysis. Using the samples, a culturing approach was implemented to enrich anaerobic organisms using a defined medium that would allow for organisms to be grown under identical conditions in future physiological comparisons. Anaerobic microorganisms were isolated and deposited with the DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) culture collection to make them available to other scientists. In MASE, the selected organisms are studied with respect to survival and growth under Mars relevant stresses. They are artificially fossilized and the resulting biosignatures studied and used to investigate the efficacy of life detection instrumentation for planetary missions. Some of the organisms belong to genera with medical and environmental importance such as Yersinia spp., illustrating how astrobiology field research can be used to increase the availability of microbial isolates for applied terrestrial purposes.

Item URL in elib:https://elib.dlr.de/113573/
Document Type:Article
Title:Anaerobic microorganisms in astrobiological analogue environments: from field site to culture collection
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Cockell, C. S.UK Centre for Astrobiology, University of Edinburgh, Edinburgh EH9 3FD, UK e-mail: c.s.cockell (at) ed.ac.ukUNSPECIFIED
Schwendner, P.UK Centre for Astrobiology, University of Edinburgh, Edinburgh EH9 3FD, UKUNSPECIFIED
Perras, A.University of Graz, Graz, Austria and Department for Microbiology, University of Regensburg, Universitätsstr. 31, 93053 Regensburg, GermanyUNSPECIFIED
Rettberg, Petraradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Beblo-Vranesevic, Kristinaradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyhttps://orcid.org/0000-0002-4834-7121
Bohmeier, M.radiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germanyUNSPECIFIED
Rabbow, Elkeradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany.https://orcid.org/0000-0002-9301-2021
Moissl-Eichinger, C.University of Graz, Graz, AustriaUNSPECIFIED
Wink, L.University of Graz, Graz, AustriaUNSPECIFIED
Marteinsson, V.Matis ohf., Reykjavik, IcelandUNSPECIFIED
Vannier, P.Matis ohf., Reykjavik, IcelandUNSPECIFIED
Gomez, F.Centro de Astrobiologia (INTA-CSIC), Torrejon de Aredoz, SpainUNSPECIFIED
Garcia-Descalzo, L.Centro de Astrobiologia (INTA-CSIC), Torrejon de Aredoz, SpainUNSPECIFIED
Ehrenfreund, P.Leiden Observatory, Leiden University, Leiden, The NetherlandsUNSPECIFIED
Monaghan, E.P.Leiden Observatory, Leiden University, Leiden, The NetherlandsUNSPECIFIED
Westall, F.Centre National de La Recherche Scientifique (CNRS), Orleans, FranceUNSPECIFIED
Gaboyer, F.Centre National de La Recherche Scientifique (CNRS), Orleans, FranceUNSPECIFIED
Amils, R.Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, Cantoblanco 28049, Madrid, SpainUNSPECIFIED
Malki, M.Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, Cantoblanco 28049, Madrid, SpainUNSPECIFIED
Pukall, R.Leibniz-Institute, DSMZ(Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), Braunschweig, GermanyUNSPECIFIED
Cabezas, P.European Science Foundation, Strasbourg, FranceUNSPECIFIED
Walter, N.European Science Foundation, Strasbourg, FranceUNSPECIFIED
Date:2017
Journal or Publication Title:International Journal of Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1017/S1473550417000246
Page Range:pp. 1-15
Publisher:Cambridge University Press
ISSN:1473-5504
Status:Published
Keywords:anaerobic samples, analogues, extremophiles, isolation, Mars, model organisms
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:16 Aug 2017 14:13
Last Modified:06 Sep 2019 15:15

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