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Kombucha Multimicrobial Community under Simulated Spaceflight and Martian Conditions

Podolich, O. and Zaets, I. and Kukharenko, O. and Orlovska, I. and Reva, O. and Khirunenko, L. and Sosnin, M. and Haidak, A. and Shpylova, S. and Rabbow, Elke and Skoryk, M. and Kremenskoy, M. and Demets, R. and Kozyrovska, N. and de Vera, J.-P (2017) Kombucha Multimicrobial Community under Simulated Spaceflight and Martian Conditions. Astrobiology, 17 (5), pp. 459-469. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2016.1480 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2016.1480

Abstract

Kombucha microbial community (KMC) produces a cellulose-based biopolymer of industrial importance and a probiotic beverage. KMC-derived cellulose-based pellicle film is known as a highly adaptive microbial macrocolony—a stratified community of prokaryotes and eukaryotes. In the framework of the multipurpose international astrobiological project ‘‘BIOlogy and Mars Experiment (BIOMEX),’’ which aims to study the vitality of prokaryotic and eukaryotic organisms and the stability of selected biomarkers in low Earth orbit and in a Mars-like environment, a cellulose polymer structural integrity will be assessed as a biomarker and biotechnological nanomaterial. In a preflight assessment program for BIOMEX, the mineralized bacterial cellulose did not exhibit significant changes in the structure under all types of tests. KMC members that inhabit the cellulose-based pellicle exhibited a high survival rate; however, the survival capacity depended on a variety of stressors such as the vacuum of space, a Mars-like atmosphere, UVC radiation, and temperature fluctuations. The critical limiting factor for microbial survival was high-dose UV irradiation. In the tests that simulated a 1- year mission of exposure outside the International Space Station, the core populations of bacteria and yeasts survived and provided protection against UV; however, the microbial density of the populations overall was reduced, which was revealed by implementation of culture-dependent and culture-independent methods. Reduction of microbial richness was also associated with a lower accumulation of chemical elements in the cellulose-based pellicle film, produced by microbiota that survived in the post-test experiments, as compared to untreated cultures that populated the film.

Item URL in elib:https://elib.dlr.de/112857/
Document Type:Article
Title:Kombucha Multimicrobial Community under Simulated Spaceflight and Martian Conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Podolich, O.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Zaets, I.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Kukharenko, O.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Orlovska, I.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Reva, O.Pretoria University, Bioinformatics Center, Pretoria, South Africa.UNSPECIFIED
Khirunenko, L.Institute of Physics of NASU, Kyiv, Ukraine.UNSPECIFIED
Sosnin, M.Institute of Physics of NASU, Kyiv, Ukraine.UNSPECIFIED
Haidak, A.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Shpylova, S.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
Rabbow, Elkeradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany.https://orcid.org/0000-0002-9301-2021
Skoryk, M.NanoMedTech LLC, Kyiv, Ukraine.UNSPECIFIED
Kremenskoy, M.NanoMedTech LLC, Kyiv, Ukraine.UNSPECIFIED
Demets, R.ESA/ESTEC, Noordwijk, the NetherlandsUNSPECIFIED
Kozyrovska, N.Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine.UNSPECIFIED
de Vera, J.-PInstitute of Planetary Research, German Aerospace Center (DLR), Berlin, Germany.https://orcid.org/0000-0002-9530-5821
Date:2017
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:17
DOI :10.1089/ast.2016.1480
Page Range:pp. 459-469
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:BIOlogy and Mars Experiment (BIOMEX), Kombucha multimicrobial community, Biosignature, Biofilm, Bacterial cellulose
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie, R - Vorhaben Exploration des Sonnensystems
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Planetary Research > Leitungsbereich PF
Deposited By: Kopp, Kerstin
Deposited On:29 Jun 2017 15:40
Last Modified:06 Sep 2019 15:23

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