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Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects

Becker, Ina and Strauch, Sebastian M. and Hauslage, Jens and Lebert, Michael (2017) Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects. Life Sciences in Space Research (13), pp. 12-18. Elsevier. DOI: 10.1016/j.lssr.2017.02.001 ISSN 2214-5524

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Official URL: http://dx.doi.org/10.1016/j.lssr.2017.02.001

Abstract

The unicellular freshwater flagellate Euglena gracilis has a highly developed sensory system. The cells use different stimuli such as light and gravity to orient themselves in the surrounding medium to find areas for optimal growth. Due to the ability to produce oxygen and consume carbon dioxide, Euglena is a suitable candidate for life support systems. Participation in a long-term space experiment would allow for the analysis of changes and adaptations to the new environment, and this could bring new insights into the mechanism of perception of gravity and the associated signal transduction chain. For a molecular analysis of transcription patterns, an automated system is necessary, capable of performing all steps from taking a sample, processing it and generating data. One of the developmental steps is to find long-term stable reagents and materials and test them for stability at higher-than-recommended temperature conditions during extended storage time. We investigated the usability of magnetic beads in an Euglena specific lysis buffer after addition of the RNA stabilizer Dithiothreitol over 360 days and the lysis buffer with the stabilizer alone over 455 days at the expected storage temperature of 19 °C. We can claim that the stability is not impaired at all after an incubation period of over one year. This might be an interesting result for researchers who have to work under non-standard lab conditions, as in biological or medicinal fieldwork.

Item URL in elib:https://elib.dlr.de/111468/
Document Type:Article
Title:Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Becker, InaFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, GermanyUNSPECIFIED
Strauch, Sebastian M.Friedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0433-5874
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Lebert, MichaelFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0448-8117
Date:18 February 2017
Journal or Publication Title:Life Sciences in Space Research
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1016/j.lssr.2017.02.001
Page Range:pp. 12-18
Publisher:Elsevier
ISSN:2214-5524
Status:Published
Keywords:Euglena gracilis, Stability, Magnetic beads, Lysis buffer, Dithiothreitol
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Project eu:cropis
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:25 Apr 2017 11:27
Last Modified:31 Jul 2019 20:08

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