Becker, Ina and Strauch, Sebastian M. and Hauslage, Jens and Lebert, Michael (2017) Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects. Life Sciences in Space Research, 13, pp. 12-18. Elsevier. doi: 10.1016/j.lssr.2017.02.001. ISSN 2214-5524.
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Official URL: http://dx.doi.org/10.1016/j.lssr.2017.02.001
Abstract
The unicellular freshwater flagellate Euglena gracilis has a highly developed sensory system. The cells use different stimuli such as light and gravity to orient themselves in the surrounding medium to find areas for optimal growth. Due to the ability to produce oxygen and consume carbon dioxide, Euglena is a suitable candidate for life support systems. Participation in a long-term space experiment would allow for the analysis of changes and adaptations to the new environment, and this could bring new insights into the mechanism of perception of gravity and the associated signal transduction chain. For a molecular analysis of transcription patterns, an automated system is necessary, capable of performing all steps from taking a sample, processing it and generating data. One of the developmental steps is to find long-term stable reagents and materials and test them for stability at higher-than-recommended temperature conditions during extended storage time. We investigated the usability of magnetic beads in an Euglena specific lysis buffer after addition of the RNA stabilizer Dithiothreitol over 360 days and the lysis buffer with the stabilizer alone over 455 days at the expected storage temperature of 19 °C. We can claim that the stability is not impaired at all after an incubation period of over one year. This might be an interesting result for researchers who have to work under non-standard lab conditions, as in biological or medicinal fieldwork.
Item URL in elib: | https://elib.dlr.de/111468/ | ||||||||||||||||||||
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Document Type: | Article | ||||||||||||||||||||
Title: | Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects | ||||||||||||||||||||
Authors: |
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Date: | 18 February 2017 | ||||||||||||||||||||
Journal or Publication Title: | Life Sciences in Space Research | ||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||
Open Access: | Yes | ||||||||||||||||||||
Gold Open Access: | No | ||||||||||||||||||||
In SCOPUS: | Yes | ||||||||||||||||||||
In ISI Web of Science: | Yes | ||||||||||||||||||||
Volume: | 13 | ||||||||||||||||||||
DOI: | 10.1016/j.lssr.2017.02.001 | ||||||||||||||||||||
Page Range: | pp. 12-18 | ||||||||||||||||||||
Publisher: | Elsevier | ||||||||||||||||||||
ISSN: | 2214-5524 | ||||||||||||||||||||
Status: | Published | ||||||||||||||||||||
Keywords: | Euglena gracilis, Stability, Magnetic beads, Lysis buffer, Dithiothreitol | ||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||
DLR - Research theme (Project): | R - Project eu:cropis (old) | ||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Gravitational Biology | ||||||||||||||||||||
Deposited By: | Duwe, Helmut | ||||||||||||||||||||
Deposited On: | 25 Apr 2017 11:27 | ||||||||||||||||||||
Last Modified: | 22 May 2024 08:29 |
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