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Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects

Becker, Ina und Strauch, Sebastian M. und Hauslage, Jens und Lebert, Michael (2017) Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects. Life Sciences in Space Research, 13, Seiten 12-18. Elsevier. doi: 10.1016/j.lssr.2017.02.001. ISSN 2214-5524.

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Offizielle URL: http://dx.doi.org/10.1016/j.lssr.2017.02.001

Kurzfassung

The unicellular freshwater flagellate Euglena gracilis has a highly developed sensory system. The cells use different stimuli such as light and gravity to orient themselves in the surrounding medium to find areas for optimal growth. Due to the ability to produce oxygen and consume carbon dioxide, Euglena is a suitable candidate for life support systems. Participation in a long-term space experiment would allow for the analysis of changes and adaptations to the new environment, and this could bring new insights into the mechanism of perception of gravity and the associated signal transduction chain. For a molecular analysis of transcription patterns, an automated system is necessary, capable of performing all steps from taking a sample, processing it and generating data. One of the developmental steps is to find long-term stable reagents and materials and test them for stability at higher-than-recommended temperature conditions during extended storage time. We investigated the usability of magnetic beads in an Euglena specific lysis buffer after addition of the RNA stabilizer Dithiothreitol over 360 days and the lysis buffer with the stabilizer alone over 455 days at the expected storage temperature of 19 °C. We can claim that the stability is not impaired at all after an incubation period of over one year. This might be an interesting result for researchers who have to work under non-standard lab conditions, as in biological or medicinal fieldwork.

elib-URL des Eintrags:https://elib.dlr.de/111468/
Dokumentart:Zeitschriftenbeitrag
Titel:Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Becker, InaFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Strauch, Sebastian M.Friedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0433-5874NICHT SPEZIFIZIERT
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000NICHT SPEZIFIZIERT
Lebert, MichaelFriedrich-Alexander-University Erlangen-Nuernberg, Department of Cell Biology, Erlangen, Germanyhttps://orcid.org/0000-0002-0448-8117NICHT SPEZIFIZIERT
Datum:18 Februar 2017
Erschienen in:Life Sciences in Space Research
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:13
DOI:10.1016/j.lssr.2017.02.001
Seitenbereich:Seiten 12-18
Verlag:Elsevier
ISSN:2214-5524
Status:veröffentlicht
Stichwörter:Euglena gracilis, Stability, Magnetic beads, Lysis buffer, Dithiothreitol
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Projekt eu:cropis (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Hinterlegt von: Duwe, Helmut
Hinterlegt am:25 Apr 2017 11:27
Letzte Änderung:22 Mai 2024 08:29

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