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The first space-related study of a Kombucha multimicrobial cellulose-forming Community: preparatory laboratory experiments

Podolich, O. and Zaets, I. and Kukharenko, O. and Orlovska, I. and Reva, O.M. and Khirunenko, L. and Sosnin, M. and Haidak, A. and Shpylova, S. and Rohutskyy, I. and Kharina, A. and Skoryk, M. and Kremenskoy, M. and Klymchuk, D. and Demets, R. and de Vera, Jean Pierre Paul and Kozyrovska, N. (2016) The first space-related study of a Kombucha multimicrobial cellulose-forming Community: preparatory laboratory experiments. Origins of Life and Evolution of Biospheres. Springer. doi: 10.1007/s11084-016-9483-4. ISSN 0169-6149.

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Official URL: http://link.springer.com/article/10.1007/s11084-016-9483-4

Abstract

Biofilm-forming microbial communities are known as the most robust assemblages that can survive in harsh environments. Biofilm-associated microorganisms display greatly increased resistance to physical and chemical adverse conditions, and they are expected to be the first form of life on Earth or anywhere else. Biological molecules synthesized by biofilm -protected microbiomes may serve as markers of the nucleoprotein life. We offer a new experimental model, a kombucha multimicrobial culture (KMC), to assess a structural integrity of a widespread microbial polymer – cellulose – as a biosignature of bacteria-producers for the multipurpose international project “BIOlogical and Mars Experiment (BIOMEX)”, which aims to study the vitality of pro- and eukaryotic organisms and the stability of organic biomolecules in contact with minerals to analyze the detectability of life markers in the context of a planetary background. In this study, we aimed to substantiate the detectability of mineralized cellulose with spectroscopy and to study the KMC macrocolony phenotype stability under adverse conditions (UV, excess of inorganics etc.). Cellulose matrix of the KMC macrocolony has been mineralized in the mineral-water interface under assistance of KMC-members. Effect of bioleached ions on the cellulose matrix has been visible, and the FT-IR spectrum proved changes in cellulose structure. However, the specific cellulose band vibration, confirming the presence of β(1,4)-linkages between monomers, has not been quenched by secondary minerals formed on the surface of pellicle. The cellulose-based KMC macrocolony phenotype was in a dependence on extracellular matrix components (ionome, viriome, extracellular membrane vesicles), which provided its integrity and rigidness in a certain extent under impact of stressful factors.

Item URL in elib:https://elib.dlr.de/111257/
Document Type:Article
Title:The first space-related study of a Kombucha multimicrobial cellulose-forming Community: preparatory laboratory experiments
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Podolich, O.Institute of Molecular Biology and Genetics of NASU, Acad. Zabolotnoho str., 150, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Zaets, I.institute of molecular biology&genetics, national academy of sciences of ukraine, kyivUNSPECIFIEDUNSPECIFIED
Kukharenko, O.institute of molecular biology & genetics of national academy of sciences of ukraineUNSPECIFIEDUNSPECIFIED
Orlovska, I.institute of molecular biology&genetics, national academy of sciences of ukraine, kyivUNSPECIFIEDUNSPECIFIED
Reva, O.M.department of biochemistry, bioinformatics and computational biology unit, university of pretoria, south africaUNSPECIFIEDUNSPECIFIED
Khirunenko, L.institute of physics of national academy of sciences, kiev, ukraineUNSPECIFIEDUNSPECIFIED
Sosnin, M.institute of physics of national academy of sciences, kiev, ukraineUNSPECIFIEDUNSPECIFIED
Haidak, A.Institute of Biology, Kyiv National Taras Shevchenko University, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Shpylova, S.institute of molecular biology & genetics of national academy of sciences of ukraineUNSPECIFIEDUNSPECIFIED
Rohutskyy, I.Institute of Physics of NASU, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Kharina, A.Institute of Biology, Kyiv National Taras Shevchenko University, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Skoryk, M.NanoTechMed LLC, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Kremenskoy, M.NanoTechMed LLC, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Klymchuk, D.Institute of Botany of NASU, Kyiv, UkraineUNSPECIFIEDUNSPECIFIED
Demets, R.esa (european space agency),estec, noordwijk, the netherlandsUNSPECIFIEDUNSPECIFIED
de Vera, Jean Pierre PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kozyrovska, N.institute of molecular biology&genetics of national academy of sciences, kyiv, ukraineUNSPECIFIEDUNSPECIFIED
Date:30 March 2016
Journal or Publication Title:Origins of Life and Evolution of Biospheres
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI:10.1007/s11084-016-9483-4
Publisher:Springer
ISSN:0169-6149
Status:Published
Keywords:Biology and Mars Experiments (BIOMEX); Kombucha; biosignature; biofilm; bacterial cellulose
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Space Exploration
DLR - Research theme (Project):R - Vorhaben Planetary Evolution and Life (old)
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Deposited By: de Vera, Dr. Jean Pierre Paul
Deposited On:15 Mar 2017 07:18
Last Modified:06 Sep 2019 15:29

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