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The Impact of Hypergravity and Vibration on Gene and Protein Expression of Thyroid Cells

Wehland, Markus and Warnke, Elisabeth and Frett, Timo and Hemmersbach, Ruth and Hauslage, Jens and Ma, Xiao and Aleshcheva, Ganna and Pietsch, Jessica and Bauer, Johann and Grimm, Daniela (2016) The Impact of Hypergravity and Vibration on Gene and Protein Expression of Thyroid Cells. Microgravity Science and Technology, 28 (3), pp. 261-274. Springer. DOI: 10.1007/s12217-015-9474-5 ISSN 0938-0108

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Official URL: http://link.springer.com/article/10.1007/s12217-015-9474-5

Abstract

Experiments in space either on orbital missions on-board the ISS, or in suborbital missions using sounding rockets, like TEXUS as well as parabolic flight campaigns are still the gold standard to achieve real microgravity conditions in the field of gravitational biology and medicine. However, during launch, and in flight, hypergravity and vibrations occur which might interfere with the effects of microgravity. It is therefore important to know these effects and discriminate them from the microgravity effects. This can be achieved by ground-based facilities like centrifuges or vibration platforms. Recently, we have conducted several experiments with different thyroid cancer cell lines. This study, as part of the ESA-CORA-GBF 2010-203 project, focused on the influence of vibration and hypergravity on benign human thyroid follicular epithelial cells (Nthy-ori 3-1 cell line). Gene and in part protein expression regulation under both conditions were analyzed for VCAN, ITGA10, ITGB1, OPN, ADAM19, ANXA1, TNFA, ABL2, ACTB, PFN2, TLN1, EZR, RDX, MSN, CTGF, PRKCA, and PRKAA1 using quantitative real-time PCR and Western Blot. We found that hypergravity and vibration affected genes and proteins involved in the extracellular matrix, the cytoskeleton, apoptosis, cell growth and signaling. Vibration always led to a down-regulation, whereas hypergravity resulted in a more heterogeneous expression pattern. Overall we conclude that both conditions can influence gene regulation and production of various genes and proteins. As a consequence, it is important to perform control experiments on hypergravity and vibration facilities in parallel to flight experiments.

Item URL in elib:https://elib.dlr.de/110565/
Document Type:Article
Title:The Impact of Hypergravity and Vibration on Gene and Protein Expression of Thyroid Cells
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Wehland, Markusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Warnke, Elisabethotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Frett, Timogerman aerospace center (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyUNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Ma, XiaoUniversity Aarhus, Department of Biomedicine and Pharmacology, Aarhus, DenmarkUNSPECIFIED
Aleshcheva, Gannaotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Pietsch, Jessicaotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Bauer, Johannmax-planck institute for biochemistry, martinsried, germanyUNSPECIFIED
Grimm, DanielaUniversity Aarhus, Department of Biomedicine and Pharmacology, Aarhus, DenmarkUNSPECIFIED
Date:14 April 2016
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:28
DOI :10.1007/s12217-015-9474-5
Page Range:pp. 261-274
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:microgravity, thyroid cells, vibration, hypergravity, cytoskeleton, integrins, apoptosis signaling, cell adhesion
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Operationelle Forschung Kurzarmzentrifuge
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:12 Jan 2017 09:01
Last Modified:12 Jan 2017 09:01

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