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An improved protocol for harvesting Bacillus subtilis colony biofilms

Fuchs, Felix Matthias and Driks, Adam and Setlow, Peter and Moeller, Ralf (2017) An improved protocol for harvesting Bacillus subtilis colony biofilms. Journal of Microbiological Methods. Elsevier. DOI: 10.1016/j.mimet.2017.01.002 ISSN 0167-7012

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.mimet.2017.01.002

Abstract

Bacterial biofilms cause severe problems in medicine and industry due to the high resistance to disinfectants and environmental stress of organismswithin biofilms. Addressing challenges caused by biofilms requires full understanding of the underlying mechanisms for bacterial resistance and survival in biofilms. However, such work is hampered by a relative lack of systems for biofilm cultivation that are practical and reproducible. To address this problem, we developed a readily applicable method to culture Bacillus subtilis biofilms on a membrane filter. The method results in biofilms with highly reproducible characteristics, and which can be readily analyzed by a variety of methods with little further manipulation. This biofilm preparation method simplifies routine generation of B. subtilis biofilms for molecular and cellular analysis, and could be applicable to other microbial systems.

Item URL in elib:https://elib.dlr.de/110451/
Document Type:Article
Title:An improved protocol for harvesting Bacillus subtilis colony biofilms
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fuchs, Felix MatthiasGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Linder Hoehe, Cologne (Köln), GermanyUNSPECIFIED
Driks, AdamLoyola University Chicago, Department of Microbiology and Immunology, and the Infectious Disease and Immunology Research Institute, Stritch School of Medicine, Maywood, IL, USAUNSPECIFIED
Setlow, PeterUniversity of Connecticut Health Center, Department of Molecular Biology and Biophysics, Farmington, CT, USA.UNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Linder Hoehe, Cologne (Köln), GermanyUNSPECIFIED
Date:2017
Journal or Publication Title:Journal of Microbiological Methods
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1016/j.mimet.2017.01.002
Publisher:Elsevier
ISSN:0167-7012
Status:Published
Keywords:Biofilm, Bacillus subtilis, Membrane filter, Spores, Quantification Reproducibility
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:09 Feb 2017 14:20
Last Modified:09 Feb 2017 14:20

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