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VUV absorption spectroscopy of bacterial spores and DNA components

Fiebrandt, Marcel and Lackmann, Jan-Wilm and Raguse, Marina and Moeller, Ralf and Awakowicz, Peter and Stapelmann, Katharina (2017) VUV absorption spectroscopy of bacterial spores and DNA components. Plasma Physics and Controlled Fusion, 59 (1), 014010. Institute of Physics (IOP) Publishing. DOI: 10.1088/0741-3335/59/1/014010 ISSN 0741-3335

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Official URL: http://dx.doi.org/10.1088/0741-3335/59/1/014010

Abstract

Low-pressure plasmas can be used to inactivate bacterial spores and sterilize goods for medical and pharmaceutical applications. A crucial factor are damages induced by UV and VUV radiation emitted by the plasma. To analyze inactivation processes and protection strategies of spores, absorption spectra of two B. subtilis strains are measured. The results indicate, that the inner and outer coat of the spore significantly contribute to the absorption of UV-C and also of the VUV, protecting the spore against radiation based damages. As the sample preparation can significantly influence the absorption spectra due to salt residues, the cleaning procedure and sample deposition is tested for its reproducibility by measuring DNA oligomers and pUC18 plasmid DNA. The measurements are compared and discussed with results from the literature, showing a strong decrease of the salt content enabling the detection of absorption structures in the samples.

Item URL in elib:https://elib.dlr.de/107979/
Document Type:Article
Title:VUV absorption spectroscopy of bacterial spores and DNA components
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fiebrandt, MarcelFaculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, Institute of Electrical Engineering and Plasma Technology, Bochum, Germany; fiebrandt (at) aept.rub.deUNSPECIFIED
Lackmann, Jan-WilmFaculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, Institute of Electrical Engineering and Plasma Technology, Bochum, Germany; Ruhr-University Bochum, Institute of Electrical Engineering and Plasma Technology, BochumUNSPECIFIED
Raguse, MarinaRadiation Biology Department, Space Microbiology Research Group, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Cologne (Koeln), GermanyUNSPECIFIED
Moeller, RalfRadiation Biology Department, Space Microbiology Research Group, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Cologne (Koeln), GermanyUNSPECIFIED
Awakowicz, PeterFaculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, Institute of Electrical Engineering and Plasma Technology, Bochum, GermanyUNSPECIFIED
Stapelmann, KatharinaRuhr-University Bochum, Institute of Electrical Engineering and Plasma Technology, Biomedical Applications of Plasma Technology, Bochum, GermanyUNSPECIFIED
Date:2017
Journal or Publication Title:Plasma Physics and Controlled Fusion
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:59
DOI :10.1088/0741-3335/59/1/014010
Page Range:014010
Publisher:Institute of Physics (IOP) Publishing
ISSN:0741-3335
Status:Published
Keywords:VUV spectroscopy, absorption, bacterial spores, DNA, Bacillus subtilis
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:04 Jan 2017 16:02
Last Modified:08 Mar 2018 18:39

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